大豆花叶病毒侵染初期抑制消减文库构建及初步分析  

Construction and primary analysis of subtractive library induced by soybean mosaic virus at the initial stage of infection

作　　者：吴艳艳[1] 张学玲[1] 徐伟[1] 蔡春梅[1] 

机构地区：[1]青岛农业大学生命科学学院,山东省高校植物生物技术重点实验室,山东青岛266109

出　　处：《延边大学农学学报》2014年第3期199-203,共5页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金资助项目(31071444;31371647);山东省优秀中青年科学家科研奖励基金(BS2011NY006);青岛农业大学高层次人才启动基金(630908;631304)

摘　　要：大豆花叶病毒病是危害大豆产量和品质的重要病害之一,通过分子手段研究SMV抗性相关基因可以辅助抗病育种工作。本研究以感病品系Sowonkong和它的抗病近等基因系（Near-isogenic line,NIL）Suwon243为材料,利用抑制差减杂交技术构建了一个大豆花叶病毒接种初期的cDNA文库。随机挑取50个阳性克隆测序,获得高质量的EST 37个。所得EST核苷酸长度分布在126~1 003bp。功能已知基因的EST涉及抗病与防御、能量和初级代谢、蛋白质合成和加工转录修饰、信号转导相关基因,其中：过氧化氢酶片段长度最长达1 003bp。Soybean mosaic virus（SMV）is one of main diseases of soybean,which could affect yields and quality seriously.Studying the genes related with SMV resistance with molecular technology is helpful to enhancing the disease resistant breeding in soybean.In this study,a soybean susceptible line,Sowonkong,and its resistant near isogenic line,Suwon243,were used to construct a subtractive cDNA library by suppression subtractive hybridization from soybean leaves inoculated by SMV at the initial stage of infection.Fifty clones were randomly picked up and sequenced,subsequently,37 high quality sequences were obtained.The length of EST fragment varied form 126-1003 bp.ESTs with known function involved in restricting virus and protection,energy and primary metabolism,protein synthesis and transcription,signal transduction.Among these,the sequence of the gene coding catalase was the longest with 1 003 bp.

关 键 词：大豆 大豆花叶病毒 抑制消减杂交 

分 类 号：Q786[生物学—分子生物学]