木薯蔗糖合酶(SuSy)基因的表达分析及SuSy1和SuSy4编码序列的克隆  

Expression Analysis of Sucrose Synthase in Cassava and Cloning of SuSy1 and SuSy4 Coding Sequences

作　　者：方开星[1,2] 陈新[2] 王海燕[2] 王淑娟[1,2] 马平安[1] 王文泉[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2014年第10期1937-1943,共7页Chinese Journal of Tropical Crops

基　　金：国家973项目课题木薯淀粉高效累积途径及其关键基因(No.2010CB126601);国家产业体系:国家木薯产业技术体系生物技术岗(No.CARS-12)

摘　　要：依据拟南芥6个蔗糖合酶基因序列搜索木薯基因组数据库,获得了6个木薯蔗糖合酶基因亚型。将6个木薯蔗糖合酶基因亚型的外显子-内含子结构进行分析,结合其它物种蔗糖合酶基因的氨基酸序列构建进化树,将木薯蔗糖合酶基因可分为三类,分别为Su Sy1/Su Sy4,Su Sy2/Su Sy3和Su Sy5/Su Sy6。以木薯KU50的功能叶和5个不同时期块根的RNA为模板,利用RT-PCR的方法对蔗糖合酶基因家族进行表达分析,确定了Su Sy1和Su Sy4高表达的亚型,克隆并获得了Su Sy1和Su Sy4基因的编码区序列,对获得的序列进行同源性和功能结构域分析表明,2条序列的氨基酸同源性为97%,并且有相同的功能结构域。According to the six subunit sequences of sucrose synthase gene in Arabidopsis,six sucrose synthase genes were identified in cassava by searching the cassava genome database.The exon-intron structures of the six sucrose synthases in cassava were analyzed and the evolutiontree was then constructed based on the sucrose synthase protein sequences from various species which divided the sucrose synthase genes into three subgroups：Su Sy1/Su Sy4,Su Sy2/Su Sy3,and Su Sy5/Su Sy6,respectively.Expression analysis of six different Su Sy subunits through RT-PCR in cassava KU50 function leave and roots of five different periods,the result showed that Su Sy1 and Su Sy4 were high expression subunits.And then,the full-length CDS of Su Sy1 and Su Sy4 were cloned and the homology and functional domain were analysis,the results showed that the homology between two sequences were97% and two sequences had the same functional domain.

关 键 词：木薯 蔗糖合酶 RT-PCR 基因克隆 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]