番茄SlARF2基因表达模式及其在果实发育中的功能分析  

The Expression Pattern of SlARF2 in Tomato and the Function Analysis in Tomato Fruit Development

作　　者：杨枭[1] 任振新[1] 林冬波[1] 先志强[1] 李正国[1] 

机构地区：[1]重庆大学生命科学学院基因工程研究中心重庆市高校功能基因与调控新技术重点实验室,重庆400030

出　　处：《热带作物学报》2014年第10期1969-1974,共6页Chinese Journal of Tropical Crops

基　　金：国家863课题(No.2012AA101702);国家自然科学基金(No.31272166);重庆市重点自然科学基金项目(No.2011BA1024);教育部博士点基金(No.20110191110029)

摘　　要：为了分析Sl ARF2基因在番茄生长发育过程中的功能,利用定量PCR技术分析Sl ARF2基因的表达模式,发现Sl ARF2基因在番茄花芽中表达量最高,在幼果中次之,说明Sl ARF2基因可能在番茄花果发育中起着重要作用。为进一步研究Sl ARF2的功能,构建Sl ARF2基因超表达、抑制表达载体,农杆菌介导转化番茄成功获得了相应转基因植株。通过与野生型番茄植株花、果不同时期的表型进行比对分析,发现Sl ARF2基因的超表达对花的形态学特征无明显影响,但能够显著提高植株的结实率、降低果实大小和重量。说明Sl ARF2基因参与调控番茄花、果发育过程。To investigate the function of Sl ARF2 in tomato development,we analyzed the expression pattern of Sl ARF2 by Real-time PCR.We found that Sl ARF2 was highly expressed in tomato bud and immature fruit,suggesting that Sl ARF2 plays an important role in tomato flower and fruit development.Furthermore,we constructed Sl ARF2 overexpression and suppress expression vectors.By agrobacterium infection,we finally obtained the Sl ARF2 overexpression and suppress transgenetic tomatoes.Compared the transgenetic tomatoes to wild type tomato,we found that Sl ARF2 had no affection in the morphology of flower,however Sl ARF2 overexpression strongly enhanced the rape rate of tomato and decreased the size of tomato fruit.The study demonstrates that Sl ARF2 regulates the development of tomato flower and fruit,and provides basic reference for further study of Sl ARF2 function.

关 键 词：番茄 Sl ARF2 表达模式 果实发育 

分 类 号：S641.2[农业科学—蔬菜学]