枇杷果实发育过程中山梨醇含量及相关酶活性的变化  

Changes of Sorbitol Content and Related Enzymes During the Development of Loquat Fruit

作　　者：吴锦程[1] 吴毕莎[1] 陈冬倩[1] 林松泉 吴丽娟[1] 冯风桃 蔡梦华 林香[1] 

机构地区：[1]莆田学院,环境与生物工程学院,福建莆田351100

出　　处：《热带作物学报》2014年第10期1997-2001,共5页Chinese Journal of Tropical Crops

基　　金：福建省科技计划重点项目(No.2011N0028);福建省自然科学基金项目(No.2008J0071);福建省高校服务海西重点建设项目(No.2008HX02)

摘　　要：以果型和品质差异较大的黄肉枇杷（Eriobotrya japonica Lindl.）‘解放钟’和白肉枇杷‘白梨’的果实为试材,测定果实发育过程中山梨醇、果糖、葡萄糖含量及与山梨醇代谢相关酶--山梨醇脱氢酶（Sorbitol dehydrogenase,SDH）和山梨醇氧化酶（Sorbitol oxidase,SOX）活性的变化,并探讨山梨醇代谢与枇杷果实生长发育和品质形成的关系。结果表明：2个枇杷品种果实中山梨醇含量均随果实发育进程呈下降趋势,且‘白梨’枇杷果实中山梨醇含量高于‘解放钟’。在花后35-80 d,2个枇杷品种幼果中葡萄糖和果糖含量均较低且相对稳定,而花后95-140 d果实中葡萄糖和果糖含量逐渐上升,并在花后125-140 d果实成熟期急剧增加而达到峰值;果实中葡萄糖含量在2个品种间差异不显著（p〉0.05）,花后125-140 d的果实成熟期,‘白梨’果实中果糖含量显著高于‘解放钟’（p〈0.05）。果实发育过程中SDH和SOX的活性均呈先降后升的变化趋势。说明2个枇杷品种果实发育过程中山梨醇、果糖、葡萄糖含量及SDH和山SOX的活性表现出相似的变化规律,但果实山梨醇含量与SDH和SOX的活性在果实膨大期均存在显著差异（p〈0.05）,这可能是黄肉枇杷与白肉枇杷品质差异的关键。The changes of sorbitol,fructose and glucose contents and the activities of sorbitol-metabolizing enzymes,including sorbitol dehydrogenase（SDH）,sorbitol oxidase（SOX）were studied during the fruit development of ‘Baili＇ and ‘Jiefangzhong＇cultivars of loquat（Eriobotrya japonica Lindl.）.The study was carried out to explore the relationships between sorbitol metabolism and the growth,development and quality formation of loquat fruits.The results showed that sorbitol content in the two cultivars generally decreased during fruit development,and sorbitol content was higher in ‘Baili＇ than in ‘Jiefangzhong＇ loquat.In the two cultivars,young loquat fruits from 35 to 80 days after anthesis exhibited low and relatively stable contents of fructose and glucose; their contents increased gradually from 95 to 140 days after anthesis,reaching to a peak from 125 to 140 days after anthesis.The glucose contents in the developing loquat fruits were similar in the two cultivars（p 〉0.05）.The fructose content of ‘Baili＇ fruit was significantly higher than that of ‘Jiefangzhong＇ fruit during fruit maturation（p 〈0.05）.In both cultivars,the activities of SDH and SOX decreased at first,and then rised during loquat fruit development.Trends of sorbitol,fructose and glucose contents and SDH,SOX activities were similar in the two cultivars,except for sorbitol content and SDH,SOX activities at the stage of loquat fruit enlargement（p 〈0.05）.This may be the key for the formation of fruit quality in the two different loquat cultivars with yellow and white pulp.

关 键 词：枇杷 山梨醇 山梨醇脱氢酶 山梨醇氧化酶 

分 类 号：S667.3[农业科学—果树学]