Activation free energy of Zn(Ⅱ),Co(Ⅱ) binding to metallo-β-lactamase ImiS  

作　　者：Xia Yang Ya-Jun Zhou Pei He Yun-Hua Guo Cong-Jun Liu Ke-Wu Yang 

机构地区：[1]Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,College of Chemistry and Materials Science,Northwest University

出　　处：《Chinese Chemical Letters》2014年第10期1323-1326,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.21272186 and 81361138018)

摘　　要：In an effort to understand the recombination of a B2 metallo-β-lactamase（MβL）,the binding of metals to apo-ImiS was studied by isothermal titration calorimetry and fluorescence spectra.The binding of Zn（Ⅱ）,Co（Ⅱ） to apo-lmiS resulted in activation free energies △G_≠~θ values of 93.719 and 92.948 kJ mol^（-1）,respectively,and increasing of fluorescence intensity at maxima emission of 340 nm.In an effort to understand the recombination of a B2 metallo-β-lactamase（MβL）,the binding of metals to apo-ImiS was studied by isothermal titration calorimetry and fluorescence spectra.The binding of Zn（Ⅱ）,Co（Ⅱ） to apo-lmiS resulted in activation free energies △G_≠~θ values of 93.719 and 92.948 kJ mol^（-1）,respectively,and increasing of fluorescence intensity at maxima emission of 340 nm.

关 键 词：Antibiotic resistant bacteria Metallo-β-lactamases Metalloprotein recombinant Thermokinetic parameters 

分 类 号：TQ465[化学工程—制药化工]