电针内关穴对心肌痛大鼠心肌和下丘脑P2X_3受体影响的研究  

Effects of Electroacupuncture at Neiguan(PC 6) on Expression of P2X_3 Receptor of Myocardium in Myocardial Pain Rats

作　　者：严琴[1] 张英[2] 刘雨生[1] 汤园园[2] 王琼[1] 乐凯[2] 李超英[2] 朱书秀[2] 凃乾[2] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]江汉大学,湖北武汉430056

出　　处：《湖北中医药大学学报》2014年第5期5-8,共4页Journal of Hubei University of Chinese Medicine

基　　金：国家自然科学基金(81720885)

摘　　要：目的观察电针"内关"对实验性心肌痛大鼠心肌和下丘脑P2X3受体表达的影响。方法成年雄性SD大鼠28只,随机分为正常组(N组)、对照组(C组)、心肌痛组(M组)、电针组(E组),每组7只。观察大鼠的行为学变化及体重变化;苏木精-伊红(HE)染色方法观测心肌组织的变化;免疫印迹法(West blotting)分别检测大鼠心肌和下丘脑P2X3受体及β-肌动蛋白(β-actin)蛋白表达,比较各组P2X3受体蛋白相对表达量。结果 C组的体重增长较少,与N组相比有统计学意义(P<0.01);M组、E组的体重增长较N组和C组显著降低(均P<0.01);E组的体重增加比M组的明显升高(P<0.01)。M组、E组的行为学表现较N组和C组显著增加(均P<0.01);与M组相比,E组的行为学表现都明显下降(P<0.01)。N组和C组的心肌细胞排列致密;M组可见严重的心肌细胞萎缩以及代偿性肥大和大面积的结缔组织增生现象;E组可见轻微的细胞肥大和结缔组织增生。M组心肌P2X3受体蛋白表达量显著高于N组、C组(均P<0.01);E组心肌P2X3受体蛋白表达量显著低于M组(P<0.01),M组下丘脑P2X3受体蛋白表达量显著高于N组、C组(均P<0.01);E组下丘脑P2X3受体蛋白表达量显著低于M组(P<0.01)。结论电针"内关"可能对P2X3受体介导的心肌痛信息产生影响,这可能是电针"内关"镇痛的机制。Objective To investigate the effects of eleetroacupunetur at Neiguan on the expression of P2X3 receptor protein of myocardi- urn and hypothalamus in myocardial pain rats induced by ISO. Methods Sprague - Dawly weighing 180 - 250g rats were used in experi- ment. Rats were injected subcutaneously with ISO for 14 consecutive days to induce myocardial pain model. Rats were randomly divided into normal group （N group）, control group treated with saline （C group）, myocardial pain group （M group）, electroacupuncture -treated myo- cardial pain group （E group）. The behavioral and weight changes were observed after rats were executed, hematoxylin and eosin staining was used to observed the changes of myocardial tissue and the P2X3 receptor and [5 - actin protein expression in myocardium were determined by Western blotting. Results Weight gain in the C group grew less than the N group, and had statistical significance （ P 〈 0.01 ）. Weight gain in the M and E group grew less than the N and C group （P 〈0.01 ）. Weight gain in the E group was significantly higher the M group （P 〈 O. 01 ）. The behavioral changes in the M and E group were higher than the N group and the C group （P 〈 0.01 ）. The behavioral changes in the E group was less than the M group （P 〈0.01 ）. The cardiac tissues from N and the C group showed thin and compact arrangement. The car- diac tissues from M group showed severe atrophy, hypertrophy and proliferation of.fiber connective tissue. The cardiac tissues from E showed slight atrophy and vicarious hypertrophy. The P2X3 receptor protein expression of myocardium in M group was significantly higher than that in N group and C group （P 〈 0. O1 ）. The P2X3 receptor protein expressions of myocardium in E group was significantly lower than that in M group （P 〈 0.01 ）. The P2X3 receptor protein expression of hypothalamus in M group was significantly higher than that in N group and C group （ P 〈 0. 01 ）. The P2X3 receptor protein expressions of hypothalam

关 键 词：P2X3受体 心肌痛 内关 

分 类 号：R245.9[医药卫生—针灸推拿学]