恶性高温综合征患猪基因表达变化及生物信息学分析  

Differential Expression and Biological Functions of Related Genes to Porcine Malignant Hyperthermia Syndrome

作　　者：方晓敏[1] 涂枫[1] 任守文[1] 王学敏[1] 李碧侠[1] 付言峰[1] 赵为民[1] 赵芳[1] 

机构地区：[1]江苏省农科院畜牧研究所,江苏南京210014

出　　处：《华北农学报》2014年第5期54-58,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31301953);国家生猪现代产业技术体系项目(nycytx-009);江苏省自然科学基金项目(BK20131332);江苏省农业科技自主创新基金项目(CX(13)2037)

摘　　要：为揭示猪发生恶性高温综合征后基因表达改变,分析基因表达变化在疾病发展过程中的生物学意义,取恶性高温综合征病猪和健康对照猪各3头,采用表达谱基因芯片检测患猪与健康猪的基因表达差异,借助NCBI、NETWORK数据库对差异基因的功能、信号传导途径及其表达改变在猪恶性高温综合征病程发展中的意义作生物信息学分析。结果表明,高温综合征患猪较健康对照出现表达上调基因30个,下调基因108个,GO分析生物学功能聚为免疫防御调节、生化调节、有机物应激调节等几大类,富集信号传导途径26条,其中猪组织相容性复合体SLA-DMA介导的免疫防御调控通路在猪恶性高温综合征中扮演重要角色。恶性高温综合征患猪stefinA1、APOD、SLA-DMA基因的表达改变导致免疫功能紊乱影响疫病进展。To detect gene expression changes after suffering from porcine malignant hyperthermia syndrome（ pMHS） and analyze the biological significance of differential expression genes in the process of disease infection.Three pigs were used in this study as cases and controls,respectively. Gene chips was conducted to investigate gene expression difference between two groups. Gene ontology and pathway analysis were applied to determine the roles of these differentially expressed genes in pMHS by NCBI and NETWORK. 138 genes were identified in MHS pigs,including 30 up-regulated and 108 down-regulated genes,comparison with the control group. These genes were confirmed associated mainly with the regulation of immune defense,biochemistry,organic stress,etc,and involved in 26 signal pathways,of which SLA-DMA mediated immune defense pathway plays an important role in pMHS. The expression changes of stefinA1,APOD,SLA-DMA genes result in immune dysfunction,which was crucial in the pathological process of pMHS.

关 键 词：猪 恶性高温综合征 基因表达 生物信息学 

分 类 号：Q78[生物学—分子生物学]