分光光度法测定酱豆中亚硝酸盐含量  

Measuring the nitrate content by spectrophotometry in jiangdou

作　　者：卜彦林[1] 张建夫[2] 郑方圆[2] 

机构地区：[1]河南省水利水电学校,河南周口466000 [2]周口师范学院化学化工学院,河南周口466001

出　　处：《周口师范学院学报》2014年第5期81-83,共3页Journal of Zhoukou Normal University

基　　金：河南省科技攻关计划项目(No.13210230491)

摘　　要：测定酱豆中的亚硝酸盐，样品提取的最佳条件是70℃水浴中提取30～40 min ．加入2 g活性炭，能有效排除色素和维生素对亚硝酸盐测定造成的干扰．测定时显色剂的加入顺序为：先加入2 mL 的对氨基苯磺酸溶液，反应5 min后，再加入1 mL的萘乙二胺盐酸溶液，3 min后用蒸馏水定容，摇匀，静置15 min ．待显色稳定后，以试剂空白作参比，用1 cm的比色皿在538 nm处用S 22 PC 型分光光度计测定溶液的吸光度．实验的相关系数 R＝0.99678，检出限是0.01447μg/m L ．During the determination of nitrite in jiangdou ,the best condition of extracting is in 70 ℃ water for 30~45 min .The interference of pigment and vitamin C on nitrite determination could be eliminated effectively when 2 g activated carbon was added .During developer addition ,2 mL of sulfanilic acid solution and 1 mL of naphthylethy lenediamine solution after 5 min could make the color reaction more stable .And ethylenediamine after 3 min with distilled water capacity ,shake , for 15 min ,wait for color ,to make CST reagent blank ,using 1 cm of glass ,and type of S 22PC spectrophotometer determi-nation of absorbance solutions in 538 nm .The correlation coefficient R of the experiment is 0.996 78 ,and the detection limit is 0.014 47 μg/m L .

关 键 词：酱豆 亚硝酸盐 分光光度法 

分 类 号：O652[理学—分析化学]