白蚁肠道木质素分解菌、染料脱色菌的分离鉴定及产酶条件优化  

Isolation,Identification and Optimization of Fermentation Conditions of Lignin Decomposition bacterium and Decolorization bacteria in Termite Gut

作　　者：高云航[1,2] 王巍[1] 刘萌[1] 勾长龙 娄玉杰[1] 马红霞[1] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]吉林农业大学动物生产与产品质量安全教育部重点实验室,长春130118

出　　处：《上海交通大学学报（农业科学版）》2014年第5期12-19,共8页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家现代农业产业技术体系建设专项(CARS-38);公益性行业(农业)科研专项经费资助(201303091);国家自然科学基金项目(31372331);吉林省科技发展计划项目(201205058);吉林省教育厅科学技术研究项目(201358)

摘　　要：分离具有木质素分解功能的菌株,并优化其产酶条件,同时观察分离菌株对染料的脱色情况,可为有效分解利用木质素提供必要的微生物资源。利用苯胺蓝平板法从黑胸散白蚁肠道中分离到2株具有木质素分解功能的菌株PY12、MX5,经16srRNA鉴定PY12、MX5分别为肠杆菌属的霍氏肠杆菌(Enterobacter hormaechei)、芽孢杆菌属的地衣芽孢杆菌(Bacillus licheniformis)。优化后菌株PY12LiP活力提高为278U/L,菌株MX5LiP活力提高为256U/L。并研究二者对6种染料的脱色效果观察,发现菌株PY12、MX5对6种染料都有不同程度的脱色作用,但对结晶紫脱色作用较弱。To provide necessary microbial resources for catalyzing lignin,the strains decomposing lignin were isolated and the fermentation conditions was optimized,the decolorizing effects of the isolated strains were investigated.The results showed that 2 strains,named PY12 and MX5,with decomposing lignin function were isolated using Azure B method from reticulitermes chinensis snyder gut.By 16 s rRNA identification,we found PY12 and MX5 belonged to Schizophyllum commune and Irpex lacteus,respectively.After optimizing the conditions of treatment to strains,the enzyme activities of PY12 and MX5 significantly increased to 278 U/L and 256 U/L,respectively.And PY12,MX5 showed some degree of decolorizing effects on six kinds of dyes,but they have a very weak decolorizing function to crystal violet.

关 键 词：白蚁 木质素分解 16 s rRNA鉴定 产酶条件 染料脱色 

分 类 号：Q936[生物学—微生物学]