含硅磷酸钙骨水泥的理化性质及体外细胞毒性  

Physical and chemical properties of calcium phosphate cement containing silicon and invitro cytotoxicity

作　　者：张亚楠[1] 胡雁[1] 杨洪[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《分子科学学报》2014年第5期376-382,共7页Journal of Molecular Science

基　　金：河南省自然科学基金资助项目(0411031900)

摘　　要：用共沉淀反应法制备硅酸三钙(C3S),将所制备的硅酸三钙(C3S)加入到磷酸钙系骨水泥(CPC)中,制备了一种新型的硅磷酸钙骨水泥(CPSC).研究了该复合骨水泥的理化性质和体外细胞毒性.与CPC骨水泥相比,硅磷酸钙骨水泥(CPSC)的固化时间延长,添加适量的C3S可提高CPC的抗压强度;在模拟体液(SBF)浸泡设定时间后,硅磷酸钙骨水泥(CPSC)降解率增加,并且在浸泡初期,SBF的pH增加.体外细胞毒性实验结果显示:复合C3S骨水泥浸提液能促进成纤维细胞的增殖,表明硅磷酸钙骨水泥有良好的生物相容性.含C3S的磷酸钙骨水泥可作为骨组织再生的生物材料使用.Tricalcium silicate（Ca3SiO5, C3S） was synthesized by coprecipatition manner, and a calcium phosphate silicate cements（CPSC） were obtained by incorporation of tricalcium silicate into the calci- um phosphate cement（CPC）. The setting properties and in vitro cytotoxicity of the cements were stud- ied . The results indicated that setting times of the CPSC were higher than the CPC. Also, certain content of C3S may increase the compressive strength of the composite paste. Compared with CPC, the composite cement showed significantly increased in degradation rate under the soaking in simulated body fluid （SBF）, and have a higher pH in initial soaking. Additionally, CPSC possesses the ability to stimulate L929 cell proliferation, known by measuring the proliferation rate using MTT method. The results indicated that the CPSC may be used as a bioactive material for bone regeneration.

关 键 词：硅酸三钙 磷酸钙骨水泥 自固化 体外细胞毒性 

分 类 号：O61[理学—无机化学]