超高效液相色谱分析条件变化对汉黄芩苷转化因子测定的影响  

Effects of UPLC analysis conditions on the conversion factor determination of wogonoside

作　　者：周琼[1] 石剑[2] 王桂平[1] 钱芳[1] 张彥焘 夏笔军[2] 

机构地区：[1]广州医科大学卫生职业技术学院,广州510180 [2]南方医科大学药学院

出　　处：《山东医药》2014年第36期4-7,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81102506/H3110);广州医学院青年科学基金资助项目(2010A02)

摘　　要：目的：考察超高效液相色谱（ UPLC）分析条件变化对汉黄芩苷转化因子（ K）测定的影响。方法配制含汉黄芩苷、汉黄芩素（摩尔浓度比为1∶1）一系列浓度的标准溶液，经UPLC定量测得两化合物峰面积，二者比值即K值。逐个改变分析条件研究K值变化情况。结果随流动相pH值由2．5逐渐增至3．5、4．5、6．5，测得K值升高10．7％～14．6％；pH值增至8．5时两化合物出峰时间明显前移，影响分析准确性。随流动相离子强度、样品介质pH值变化，K值变化很小（偏差＜4％）。检测波长由280 nm变至254、274、342 nm时，K值随之变化（4．1％～10．7％）。结论汉黄芩苷K值测定易受某些UPLC分析条件如流动相pH值、检测波长等变化的影响。对无对照品UGT代谢产物进行定量分析时，为保证结果准确可靠，在各种样品检测过程中最好保持分析条件完全一致。Objective To investigate the effects of UPLC analysis conditions on the conversion factor （ K） determina-tion of wogonoside .Methods A series of standard working solutions containing wogonoside and wogonin （ molar ratio 1∶1 ） were prepared and determined by UPLC to obtain peak area ratio of the two compounds , which was equal to the value of K.Then, analysis conditions were altered respectively and observed the variance of K value .Results When pH value of mobile phase A rose from 2.5 to 3.5, 4.5 and 6.5, K values were found to increase 10.7%-14.6%.When the pH value rose to 8.5, the retention time of wogonoside and wogonin moved forward significantly , which affected the accuracy of K value determination .With the variance of ionic strength of mobile phase A and pH value of sample medium , the K values determined were similar （deviation〈4%）.With the variance of detection wavelength from predetermined 280 nm to 254, 274, 342 nm, deviation of K value was in the range of 4.1%-10.7%.Conclusions The K value determination of wogonoside is susceptible to the variance of several UPLC analysis conditions such as pH value of mobile phase A , detection wavelength .To provide more precise quanification for glucuronides which are usually not available commercially , it is nec-essary to keep UPLC analysis conditions consistent during the process of determination .

关 键 词：转化因子 UPLC分析条件 汉黄芩苷 汉黄芩素 

分 类 号：R969.1[医药卫生—药理学]