大鼠脊髓损伤后勿动蛋白受体的动态表达  

Dynamic Expression of Nogo Receptor after Spinal Cord Injury in Rats

作　　者：杨俊锋[1] 张亚峰[1] 马勇[2] 吴毛[1] 郭杨[2] 顾晓林[2] 王建伟[1] 

机构地区：[1]无锡市中医医院,南京中医药大学骨伤研究所,江苏无锡市214000 [2]南京中医药大学,江苏南京市210023

出　　处：《中国康复理论与实践》2014年第10期919-923,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：江苏省自然科学基金资助项目(No.BK2011180);江苏省2013年度普通高校研究生科研创新计划项目(No.CXZZ13-0614);江苏省2012年度普通高校研究生科研创新计划项目(No.CXZZ12-0609)

摘　　要：目的观察大鼠脊髓损伤后勿动蛋白受体(NgR)在脊髓组织中的动态表达变化。方法 108只Sprague-Dawley大鼠随机分成正常组、假手术组和模型组,每组36只,其中模型组采用改良Allen法造模。3组分别于干预后24 h、3 d、7 d、14 d处死大鼠,每组9只,以免疫组化及Western blotting检测各组大鼠脊髓组织中NgR表达的变化,以荧光定量PCR检测NgR mRNA表达变化。结果正常组、假手术组各时间点NgR表达无明显变化(P>0.05)。模型组在损伤后24 h NgR及其mRNA表达较低,3 d后下降至最低,7 d后迅速上升达到高峰,14 d时有所下降。与正常组比较,模型组在损伤后各时间点,NgR免疫组化及Western blotting蛋白表达差异均有统计学意义(P<0.05);与假手术组比较,模型组在损伤后各时间点,NgR mRNA表达差异均有统计学意义(P<0.01)。结论大鼠脊髓损伤后,NgR及其mRNA表达均在7 d时上升至峰值,并较长时间保持高水平表达。这可能是造成脊髓损伤后轴突再生困难的重要原因之一。Objective To observe the dynamic expression of Nogo receptor（NgR） in spinal cord of rats after spinal cord injury. Methods 108 Sprague-Dawley rats were randomly assigned into normal group, sham operated group and model group, with 36 rats in each group.The model of spinal cord injury was established with the modified Allen＇s method. The rats were killed 24 h, 3 days, 7 days and 14 days respectively after intervention（9 rats from each group）, and expression of NgR in the spinal cord tissue of the rats was detected with immunohistochemistry and Western blotting, and expression of NgR mRNA was detected with fluorescence quantitative PCR. Results There was no significant change in the expression of NgR in the normal group and the sham operated group（P〉0.05）. The expression of protein and mRNA of NgR was less in the model group 24 h after modeling, dropped to the lowest on the 3rd day, then rapidly peaked on the 7th day,and gradually declined on the 14 th day after spinal cord injury. Compared with the normal group, there were significant differences in expression of NgR in immunohistochemistry and Western blotting in the model group at each time point after spinal cord injury（P〈0.05）.Compared with the sham operated group, there were significant differences in expression of NgR mRNA in the model group at each time point after spinal cord injury（P〈0.01）. Conclusion The expression of NgR and mRNA peaks on the 7th day after spinal cord injury in the rats, and maintains at high level for a long time, which may associated with the difficulty of axonal regeneration after spinal cord injury.

关 键 词：脊髓损伤 勿动蛋白受体 轴突再生 大鼠 

分 类 号：R651.2[医药卫生—外科学]