HPLC法测定麻杏石甘散中苦杏仁苷的含量  

Determination of amygdalin in Ma Xing Shi Gan powder by HPLC

作　　者：邱天宝[1] 刘素梅[1] 方忠意[1] 班付国[1] 韩立[1] 王丽[1] 

机构地区：[1]河南省兽药监察所,河南郑州450008

出　　处：《中兽医医药杂志》2014年第5期40-42,共3页Journal of Traditional Chinese Veterinary Medicine

摘　　要：建立HPLC法测定麻杏石甘散中苦杏仁苷含量的方法。色谱柱为Waters XBrigeTM C18(4.6 mm×150 mm,5μm);以水-甲醇-乙腈(90.0∶1.0∶9.0)为流动相;流速:1.0 mL/min;柱温:40℃;检测波长:207 nm。苦杏仁苷的线性范围为1.600 8×104-8.004×104μg,R2=1.000 0,平均回收率为97.26%(RSD为0.4%)。本方法快速、简便、准确,能有效测定麻杏石甘散中苦杏仁苷的含量。To establish an HPLC method for determination of amygdalin in Ma Xing Shi Gan powder. The amygdalin was analyzed on an XBrigeTM C,s column （4.6 mm ×250 mm, 5 μm） using ultrapure water - methanol - acetonitrite （90.0：1.0：9.0） as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 40 ℃. The detection wavelength was set at 207 nm. The calibration curve was linear in the range of 1.600 8×10-4-8,004×104 μg （R2=1.000 0） for amygdalin. The recovery of amygdalin was 97.26% with RSD of 0.4%. This method is rapid, simple and accurate, which can be used for the determination of amygdalin in Ma Xing Shi Gan powder.

关 键 词：麻杏石甘散 苦杏仁苷 含量测定 HPLC 

分 类 号：S853.7[农业科学—临床兽医学]