二色补血草LbARP基因的克隆及其表达分析  

Cloning and Expression of an Auxin-repressed Protein Gene LbARP from Limonium bicolor

作　　者：刁桂萍[1] 柳燕[1] 宋佳[1] 林佳忱 刘志华[1] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2014年第32期11250-11252,共3页Journal of Anhui Agricultural Sciences

基　　金：中央高校基本科研业务费专项资金项目(2572014BA08);黑龙江省自然科学基金面上项目(C201126)

摘　　要：从二色补血草(Limonium bicolor)cDNA文库中分离出一个生长素抑制蛋白基因LbARP(GenBank登录号为KF886022)。该基因全长698 bp,含有3个外显子和2个内含子,开放读码框(ORF)387 bp,共编码128个氨基酸,推测蛋白质分子量为13.84 kDa,理论等电点为9.69。多序列比对结果显示,该基因编码的氨基酸序列与其他植物的ARP蛋白序列相似性达到50%以上,具有生长素抑制基因家族的保守结构域。荧光定量PCR结果表明,LbARP基因能够对NaCl、ABA、CuSO4、CdCl2及ZnCl2等非生物胁迫作出应答。The full length cDNA of a novel auxin repressed protein （LbARP） gene was cloned from a cDNA library of Limonium bicolor （GenBank accession No.KF886022）.The full-length cDNA sequence of LbARP is 698 bp,including three exons and two introns.The open reading frame （ORF） is 387 bp in length,encoding a deduced amino acid sequence of 128 residues with a molecular weight of 13.84 kDa and an isoelectric point of 9.69.Sequence alignment of the deduced amino acids of LbARP revealed a low degree of similarity with other members of plant ARP and had a typical domain characteristic.The results of real-time quantitative PCR showed that LbARP can response to the treatments of NaCl,ABA,CuSO4,CdCl2 and ZnCl2.

关 键 词：二色补血草 生长素抑制蛋白 实时定量PCR 

分 类 号：S567[农业科学—中草药栽培]