江苏省血吸虫病监测预警系统的研究Ⅵ基于水面日本血吸虫尾蚴富集的水体感染性检测技术  

Surveillance and forecast system of schistosomiasis in Jiangsu Province Ⅵ Detection technology of water infectivity based on enrichment of Schistosoma japonicum cercariae on water surface

作　　者：曲国立[1] 戴建荣[1] 邢云天[1] 汪伟[1] 杨振坤[1] 赵正阳[1] 郭娜[1] 孙乐平[1] 梁幼生[1] 

机构地区：[1]江苏省血吸虫病防治研究所、卫生部寄生虫病预防和控制技术重点实验室、江苏省寄生虫分子生物学重点实验室,无锡214064

出　　处：《中国血吸虫病防治杂志》2014年第5期510-513,共4页Chinese Journal of Schistosomiasis Control

基　　金：江苏省血地寄应用性课题(X201113);江苏省科技厅临床科技专项--公共卫生关键技术应用研究(SBL2014040101)

摘　　要：目的探索水面血吸虫尾蚴富集方法,并与已有疫水检测技术相结合,建立新型快速敏感的疫水检测方法。方法选取大豆油、汽油、试剂煤油、异佛尔酮等进行扩展剂筛选。借助扩展剂在水面扩散时所形成的推力,对水面漂浮的尾蚴进行富集,然后采用PE吸附膜及C-6膜粘附富集的尾蚴,快速确定水体的感染性。同时探索扩展剂剂量与扩散半径之间的关系。结果汽油、试剂煤油、异佛尔酮均可作为扩展剂进行实验,异佛尔酮的扩散效果最优。采用扩展剂富集后进行吸附膜粘取,可显著提高水中尾蚴检出率。结论新方法可以有效提高感染性疫水的检出率,适合低感染度水体检测。Objective To explore the enrichment technique of Schistosoma japonicum cercariae on the water surface,so as to establish a new method combined with the existing technology to detect the cercarial infested water body quickly and sensitively. Methods Soybean oil,gasoline,kerosene and isophorone were screened as expanding agents. The cercariae were enriched by the thrust of the expanding agents when diffusing on the water surface,and PE adsorption film and C-6 film were applied to seize them so as to determine the infectivity of the water quickly. The relationship between the dose of expanding agents and diffusion radius were explored. Results Gasoline,kerosene and isophorone were suitable expanding agents,and the diffusion effect of isophorone was the best. After the enrichment by the expanding agents,the detection rate of cercariae of the method seizing cercariae with the film significantly improved in the water. Conclusion This new method could effectively improve the detection rate of the cercarial infested water and is suitable for the low-degree infested water.

关 键 词：日本血吸虫 预警 监测 扩展剂 C-6膜 尾蚴 

分 类 号：R532.21[医药卫生—内科学]