生育三烯酚抑制结肠癌细胞增殖中Notch信号蛋白的表达及意义  

The expression and significance of Notch signaling pathway key proteins in inhibiting human colon cancer cell SW620 proliferation by δ-tocotrienol

作　　者：何宁[1] 张静姝[1] 刘英华[1] 张静[1] 姜淑卿[1] 管彤[1] 王晓军[1] 张书婧[1] 

机构地区：[1]天津市疾病预防控制中心毒理室,天津300011

出　　处：《中国慢性病预防与控制》2014年第5期552-554,共3页Chinese Journal of Prevention and Control of Chronic Diseases

基　　金：天津市卫生局科技基金(2012KY17);国家自然科学基金面上项目(30771801)

摘　　要：目的探讨δ-生育三烯酚对人结肠癌细胞SW620细胞增殖抑制作用,研究Notch信号通路中关键蛋白在δ-生育三烯酚抑制结肠癌细胞增殖中的表达及意义。方法以终浓度分别为5、10、15、20、25、30μmol/L的δ-生育三烯酚培养SW620细胞,应用噻唑蓝(MTT)试验观察δ-生育三烯酚抑制结肠癌细胞增殖的作用,计算细胞存活率。应用免疫细胞化学方法检测Notch信号通路关键蛋白Notch、Jagged的表达变化。观察浓度为1μg/ml放线菌酮(CHX)对生育三烯酚抑制结肠癌细胞增殖作用的影响。结果随着δ-生育三烯酚浓度的增加,SW620细胞的存活率逐渐降低,差异有统计学意义(P<0.05)。Notch信号通路关键蛋白Notch1、Jagged1在溶剂对照组结肠癌细胞中表达量最高,随着加入生育三烯酚剂量的增加,表达量逐渐降低。经CHX干预过的细胞,再加入δ-生育三烯酚,作用24 h后,细胞存活率(83.33%)较单独加入生育三烯酚作用的细胞存活率(28.51%)显著增高,差异有统计学意义(P<0.05)。结论生育三烯酚抑制人结肠癌细胞增殖并诱导其死亡的方式可能为paraptosis样死亡,Notch信号通路中关键蛋白参与了这一过程。Objective To study the expression and significance of Notch signaling pathway key proteins in inhibiting human colon cancer cell SW620 proliferation by δ-tocotrienol. Methods M3T assay was used to observe the inhibiting effect of human colon cancer cell SW620 proliferation induced by δ-tocotrienol （5, 10, 15, 20, 25, 30 p.mol/L） and the cell viability was calculated. The expression of Notch signaling pathway key proteins （Notch-1 and Jagged-1） was detected by immunocytochemistry. The effect of CHX （ 1 μg/ml） on inhibiting human colon cancer cell SW620 proliferation by δ-tocotrienol was observed. Results The cell viability of SW620 cells decreased significantly with increasing δ-tocotrienol concentration, as compared with control group （P〈0.05）. The expression of Notch signaling pathway key proteins Notchl and Jaggedl was reduced by δ-tocotrienol. SW620 cells interfered by CHX were exposed to δ-tocotrienol for 24 h, the cell viability （83.33%） significantly enhanced as compared with SW620 cells only exposed to δ-tocotrienol （28.51%） （P〈0.05）. Conclusion δ-tocotrienol could inhibit the human colon cancer cell SW620 proliferation and induce a paraptosis-like death. Notch signaling pathway key proteins may be involved in this process.

关 键 词：生育三烯酚 结肠癌 细胞增殖 

分 类 号：R735.35[医药卫生—肿瘤]