高效液相色谱-串联质谱法同时测定生物样品中酪氨酸及其代谢产物  

Determination of Tyrosine and Tyrosine metabolites in Biological Sample by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：冯蕾[1] 马雅倩[2] 谭婷[1,2] 鄢爱平[2] 郭岚[2] 万益群[1,2] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学分析测试中心,江西南昌330047

出　　处：《南昌大学学报（理科版）》2014年第4期363-367,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(21465017);国家科技支撑计划项目(2012BAK17B02);国家自然青年科学基金资助项目(21105043);江西省自然科学基金项目(20122BAB203013)

摘　　要：建立了高效液相色谱-串联质谱法测定乳腺癌患者血清中多巴、酪氨酸、对羟苯基乳酸和酪胺的分析方法。在已优化的质谱条件下，待测物在6 min内完成分离，且效果良好。结果表明，4种物质的检出限在0．0005～0．20 mg·L^-1之间；定量限在0．001～0．40 mg·L^-1之间；线性相关系数在0．9989～0．9997之间，线性关系较好。血清中4种待测物在3个不同加标水平下的平均回收率在85．2％～114．0％之间，相对标准偏差在0．7％～8．5％之间。以上结果表明，该方法准确可靠，可用于实际样品的测定。A simple,selective,rapid and reliable method for the simultaneous determination of 3,4-di-hydroxyphenylalanine （DOPA）,tyrosine （Tyr）,4-hydroxyphenyllactic acid （PHPLA）and 4-hydroxyphen-ethylamine （TA）in human serum by high-performance liquid chromatography （HPLC）coupled with tan-dem mass spectrometry （MS/MS）was proposed for the first time.Under optimized conditions,all com-pounds were resolved within 6 min.The calibration curves were indicative of good linearity （correlation co-efficients ＆gt;0.998 9）,and the limits of detections and quantifications for Tyr and its metabolites ranged from 0.000 5 to 0.20 mg·L^-1 and 0.001 to 0.40 mg·L^-1 ,respectively.Recoveries of Tyr and its metabo-lites in breast cancer patient were in the range of 85.2%~114.0%,with RSDs of 0.7%~8.5%.The pres-ent method was successfully applied to the determination of DOPA,Tyr,PHPLA and TA in sera from breast cancer patients.

关 键 词：高效液相色谱-串联质谱 酪氨酸 酪氨酸代谢产物 

分 类 号：O657.63[理学—分析化学]