外源ABA提高星油藤幼苗抗冷害能力的探讨  

An investigation of exogenous abscisic acid on improving chilling tolerance of the seedlings of sacha inchi( Plukenetia volubilis)

作　　者：罗银玲[1] 苏志龙[1] 崔现亮[1] 李孙洋[1] 兰芹英[2] 

机构地区：[1]普洱学院生命科学系,云南普洱665000 [2]中国科学院西双版纳热带植物园热带森林生态重点实验室,云南勐腊666303

出　　处：《云南大学学报（自然科学版）》2014年第6期936-941,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-Z-15);国家自然科学基金(31360059)

摘　　要：星油藤是热带经济作物,对冷害比较敏感.研究通过不同浓度ABA叶面喷施探讨外源ABA能否提高星油藤幼苗的抗冷害能力.在幼苗叶面喷施水或2,5,10μmol/L和50μmol/L的ABA溶液后,放在4℃条件下2 d进行冷害处理.冷害处理导致喷施水的幼苗冷害等级提高,而2～50μmol/L ABA溶液处理的幼苗冷害等级相对较低.冷害导致幼苗叶片中的脂质过氧化水平、超氧阴离子的生成速率及过氧化氢含量提高;而ABA处理降低了它们提高的水平.冷害导致叶片中抗氧化酶活性降低,ABA处理可增强超氧化物歧化酶、过氧化氢酶、和愈创木酚过氧化物酶、谷胱甘肽还原酶与脱氢抗坏血酸还原酶的活性;抗坏血酸过氧化物酶活性在ABA预处理后无显著变化.这些结果表明ABA可通过诱导星油藤的抗氧化酶活性来提高星油藤幼苗的冷害抗性.10μmol/L以下的ABA溶液处理能达到提高抗冷害能力的目的.Sacha inchi（ Plukenetia volubilis） is a commercial tropical crop,which is sensitive to chilling. The exogenous ABA was applied on the seedlings by foliage spray and the chilling tolerance was studied. The chilling treatment was done at 4 ℃ for 2 days after the seedlings were sprayed by water or ABA solutions at 2,5,10μmol /L and 50 μmol /L. Chilling increased the injury grades of the seedlings spayed by water,meanwhile the seedlings sprayed with ABA solution had a lower injury grade. Chilling led to a higher level of lipid peroxidation,production rate of super anion,and content of H2O2,and ABA application was helpful in reducing the injury grades. Chilling decreases the activities of antioxidant enzymes in sacha inchi leaves,and ABA can enhance the activity of superoxide dismutase,catalase,guaiacol peroxidase,glutathione reductase,and dehydroascorbate reductase; the activity of ascorbate peroxidase has no significant difference after ABA treatment. These results indicate that exogenous ABA can increase the chilling tolerance of sacha inchi seedlings by enhancing activities of antioxidant enzymes. ABA solution at 10 μmol / L（ and 10 μmol / L） can increase the chilling tolerance.

关 键 词：脱落酸 冷害 抗氧化系统 星油藤 

分 类 号：S565[农业科学—作物学]