双对羟基苯甲醛Schiff碱的合成及与DNA作用  

Synthesis of Bis(p-Hydroxybenzaldehyde) Schiff Base and Its Interaction with DNA

作　　者：许瑞波[1] 杨凡[1] 梁明秀[1] 曹瑞[1] 马卫兴[1] 

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005

出　　处：《精细化工》2014年第11期1376-1379,共4页Fine Chemicals

基　　金：江苏省高校自然科学研究基金(13KJB150005);江苏省博士后科研基金(1201049C)~~

摘　　要：以1,4-二(3-氨基丙基)哌嗪(BAPP)与对羟基苯甲醛(PHBA)为原料,合成了含哌嗪环的双对羟基苯甲醛Schiff碱——N,N'-二(4-羟基苄基)-1,4-二(3-亚氨基丙基)哌嗪;产物结构经元素分析、红外光谱、核磁共振氢谱和紫外光谱进行确认。采用紫外吸收光谱法和黏度法研究了标题化合物与小牛胸腺DNA(CT-DNA)的相互作用,发现紫外吸收光谱出现了减色效应和红移现象,DNA的相对黏度随着化合物浓度的增大而增加,这些现象说明该双Schiff碱以插入方式与CT-DNA结合。Bis（p-hydroxybenzaldehyde） Schiff base containing piperazine ring,N,N＇-bis（4- hydroxybenzyl）-1,4-bis（3-iminopropyl）piperazine, was synthesized with 1,4-bis（3-amino- propyl）piperazine （BAPP） and p-hydroxybenzaldehyde （PHBA） as raw materials. The structure of the product was characterized by means of elemental analysis, IR, 1HNMR and UV-vis spectra. The interaction of the title compound with CT-DNA was investigated by UV-vis absorption spectroscopy and viscosimetry. The hypochromic effect and red shift were observed in the UV-vis absorption spectra, and the relative viscosity of CT-DNA increased with the addition of the title compound. The results indicated that the bis-Schiff base binds to CT-DNA by intercalation mode.

关 键 词：1 4-二(3-氨基丙基)哌嗪 双对羟基苯甲醛Schiff碱 与CT-DNA作用 医药与日化原料 

分 类 号：TQ464.4[化学工程—制药化工]