农杆菌介导超高产大豆子叶节遗传转化研究  

Study on Agrobacterium-mediated Transformation System of Super-high-yielding Soybean Cotyledon Node

作　　者：贾钰莹[1] 蒋滢[2] 赵强[1] 谢甫绨[1] 于翠梅[1] 

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110866 [2]沈阳师范大学生化与化学与生命科学学院,辽宁沈阳110034

出　　处：《大豆科学》2014年第5期634-637,共4页Soybean Science

基　　金：转基因生物新品种培育重大专项(2013ZX08004-004-003-004)

摘　　要：为建立一个大豆子叶节高效遗传转化体系用于大豆基因工程育种,以超高产大豆品种为材料,子叶节为外植体进行了遗传转化,研究了农杆菌侵染的菌液浓度、侵染时间、共培养基中乙酰丁香酮浓度及共培养时间等影响农杆菌转化的因素。结果表明：适宜转化的侵染和共培养条件为侵染菌液浓度OD600=0.5,侵染时间30 min,共培养基含有乙酰丁香酮浓度200μmol·L^-1,共培养时间为3-4 d。并以此条件对不同基因型超高产大豆品种进行农杆菌转化,结果显示大豆基因型对农杆菌的敏感性存在显著性差异,以沈农9号最敏感,沈农12次之,中黄35最差。To establish a high frequency genetic transformation system of soybean cotyledon node for soybean genetic engineering,Agrobacterium concentration,infection time,co-cultivation time and acetosyringone concentration of co-culture medium during soybean cotyledonary node transformation were studied by using soybean cultivars with super-high-yielding. The results showed that the optimal conditions were Agrobacterium concentration OD600= 0. 5,infection time 30 min,co-cultivation time 3-4days and acetosyringone concentration 200 μmol·L-1. Using the optimized transformation procedure,different genotypes of super-high-yielding soybeans were transformed and the sensitivity of soybean genotypes to Agrobacterium tumefaciens was significant differently. Shennong 9 was the most sensitive material,followed by Shennong 12,and Zhonghuang 35 was the least sensitive material.

关 键 词：超高产大豆 根癌农杆菌 子叶节 转化 

分 类 号：S565.1[农业科学—作物学]