大豆CYS家族基因的鉴定、启动子分析及EST表达研究  

Genome-wide Identification,EST Expression,and Upstream Sequence Analysis of CYS Family Genes in Soybean

作　　者：谢翎[1,2] 金正俊 汪章勋[3] 黄勃[3] 

机构地区：[1]安庆师范学院生命科学学院,安徽安庆246011 [2]韩国国家农业科学院农业生物部 [3]安徽农业大学微生物防治省重点实验室,安徽合肥230036

出　　处：《大豆科学》2014年第5期654-660,666,共8页Soybean Science

基　　金：国家自然科学基金(31201568);安徽省高校省级自然科学项目(KJ2011B081;KJ2013B123);安庆师范学院科研启动项目(044-K05000000281)

摘　　要：利用大豆基因组数据库,通过生物信息学分析,鉴定并获得大豆所有CYS家族基因的序列、结构以及定位信息。全基因组分析检测表明,大豆染色体DNA中共含有19个CYS家族基因成员。基因定位分析表明,这些基因分别分布于大豆的12条染色体。系统进化分析将大豆的全部CYS基因分成了3个亚家族。启动子分析揭示,19个大豆CYS基因的上游调控序列都含有逆境反应顺式元件。EST表达谱分析表明,大豆CYS家族基因大多在子叶、花、叶片以及种皮等组织中优势表达。Based on bioinformatic method,the CYS family genes of soybean were identified and their sequences,structures and chromosome location were determined. CYS proteins were classified according to their structure and phylogeny relationship. The results of genome analysis showed that a total of 19 CYS genes,located on 12 chromosomes,respectively,could be systematically identified from soybean genome and classified into three classes. All of them had stressed related cis-acting elements in their upstream sequences. The EST data of CYS family genes from the UniGene database was used to analyze the expression pattern of those genes in different tissues. EST analysis revealed that there was predominant expression of most soybean CYS family genes in cotyledon,flower,leaf and seed coat. These results will facilitate functional analysis and utilization of CYS genes in soybean.

关 键 词：CYS家族基因 差异表达 进化分析 

分 类 号：S565.1[农业科学—作物学]