大豆籽粒中异黄酮及底物含量动态变化研究  

Dynamic Change of Soybean Isoflavone and Its Synthetic Substrate During Seeds Developing

作　　者：王静[1] 李冬梅[1] 黄月明[1] 李文滨[1] 张大勇[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2014年第5期697-700,共4页Soybean Science

基　　金：国家自然科学基金(31101168);哈尔滨市科技局创新人才项目(2011RFQXN040);东北农业大学博士科研启动基金项目

摘　　要：大豆异黄酮具有特定的生理活性,因此引起人们的广泛关注。研究大豆籽粒发育过程中异黄酮含量及其合成底物含量的变化规律,分析造成大豆籽粒异黄酮含量差异的合成代谢底物方面的原因,对于利用传统或分子生物学手段改良大豆籽粒异黄酮含量均具有重要意义。为此,选用大豆籽粒异黄酮含量差异较大的6份材料统一种植,在籽粒发育的R5、R6、R7和R8期分别取籽粒提取测定异黄酮、游离苯丙氨酸、香豆酸和柚皮素含量。结果表明:大豆异黄酮的3种苷元总含量及大豆黄素、染料木素含量均随生育进程逐渐升高;大豆黄素先于染料木素在大豆种子中开始积累;大豆籽粒中游离苯丙氨酸和柚皮素含量随种子发育进程含量逐渐降低,香豆酸含量逐渐升高;不同品种异黄酮含量在各生育阶段与游离苯丙氨酸含量多呈负相关,与香豆酸含量多呈正相关。The soybean isoflavone has become a worldwide focus,as it has many beneficial functions to human beings. It will be helpful to the genetic improvement of isoflavone content that analyzing isoflavone content and its synthetic substrate content during seeds developing and the reasons of the differences between isoflavone content because of substrate content. The experiment was designed for the above purpose. Six soybean lines with different isoflavone content were planted in this experiment.The content of isoflavone,free phenylalanine,coumarate and naringenin were measured at R5,R6,R7 and R8,respectively. The results showed that the content of isoflavone increased from R5 to R8,and the synthesis and accumulation of daidzein was earlier than genistein. The content of free phenylalanine and naringenin decreased from R5 to R8. The content of coumarate increased from R5 to R8. The correlation coefficients between the isoflavone content and the free phenylalanine content were nearly negative except for R7 stage. All the correlation coefficients between the isoflavone content and the coumarate content were positive. The correlation coefficients between the isoflavone content and naringenin content were a little confusing.

关 键 词：大豆异黄酮 苯丙氨酸 香豆酸 柚皮素 

分 类 号：S565.1[农业科学—作物学]