新生小鼠耳蜗基底膜体外培养观察  

Cultivation and observation of basilar membrane of cochlea from neonatal mouse in vitro

作　　者：罗颜[1] 曹忠胜[1] 刘志勇[1] 辛洁[1] 

机构地区：[1]苏州大学附属第二医院耳鼻咽喉科,江苏苏州215004

出　　处：《新乡医学院学报》2014年第10期789-791,共3页Journal of Xinxiang Medical University

基　　金：苏州市科技计划项目(编号:SYS201233);苏州市科技计划指导项目(编号:SYSD2013086)

摘　　要：目的建立新生昆明小鼠耳蜗基底膜体外培养的模型并观察耳蜗基底膜培养的组织学形态。方法取出生2~3 d的昆明小鼠耳蜗基底膜,置入24孔细胞培养板,在含有体积分数10%胎牛血清的达尔伯克改良伊格尔/F12培养基中进行培养。采用四甲基异硫氰酸罗丹明标记的鬼笔环肽荧光染色毛细胞,抗神经丝蛋白荧光染色听神经纤维及螺旋神经节。观察耳蜗毛细胞、听神经纤维及螺旋神经节的生长情况。结果耳蜗基底膜经培养6 d,基底膜保持结构完整,毛细胞和螺旋神经节生长良好。结论本培养方法简便易行,可用于耳蜗基底膜体外培养实验研究。Objective To establish the cuhivation model in vitro of basilar membrane of cochlea in neonatal Kunming mouse and observe the histological structure of basilar membrane of cochlea. Methods The basilar membrane of cochleae from Kunming mouse of postnatal 2 - 3 days was taken and positioned in 24-well plate. The specimens were cultured in dulbec- co＇s modified eagle＇s medium（ DMEM）/F12 medium which containing volume ratio 10% bovine serum albumin. Tetramethyl- rhodamine isothiocyanate-labeled phalloidine staining was used to stain hair cell, while neurofilmnent protion was used to stain auditory nerve and spiral ganglion neurons. The growth of cochlear hair cells, auditory nerve and spiral ganglion neurons were observed. Results The cochlear basilar membrane has been cultured for six days. The structure of basilar membrane was in- tact. The growths of cochlear hair cells and spiral ganglion neurons were good. Conclusion This culture method is simple and effective,which can be applied to the experiment of cochlear basilar membrane in vitro culture.

关 键 词：基底膜培养 毛细胞 螺旋神经节 

分 类 号：R322.92[医药卫生—人体解剖和组织胚胎学]