不同剂量三羟异黄酮对去势大鼠子宫质量及内膜的影响  

Effect of different doses of genistein on uterus and endometrium in ovariectomized rats

作　　者：姚守珍 郑若姮[2] 石巧鹃[3] 

机构地区：[1]杭州市妇产科医院妇产科,310000 [2]浙江省中西医结合医院生殖科 [3]浙江省医学科学院实验动物中心

出　　处：《浙江医学》2014年第18期1535-1537,1576,共4页Zhejiang Medical Journal

基　　金：浙江省中医药管理局基金(Y026B)

摘　　要：目的探讨不同剂量的植物雌激素三羟异黄酮(GST)对子宫质量及内膜的影响。方法选取50只成熟雌性SD大鼠,随机分为5组:假手术组、去卵巢组、去卵巢+GST低剂量组(25rmg/kg)、去卵巢+GST中剂量组(50mg/kg)、去卵巢+GST高剂量组(100rmg/kg),后3组每天以不同剂量GST溶液灌胃,实验持续4周。结果实验结束时,各组大鼠体质量较实验前均有增加,差异均有统计学意义(均P<0.05),但组间比较差异无统计学意义(P>0.05);GST低、中、高剂量组子宫质量分别为(0.18±0.03)、(0.16±0.04)、(0.17±0.05)g;对应的子宫质量/体质量分别为0.64±0.05、0.53±0.08、0.58±0.03,3组间的差异无统计学意义(P>0.05);分别与去卵巢组比较[子宫质量(0.15±0.05)g、子宫质量/体质量0.48±0.08],差异均无统计学意义(均P>0.05);但与假手术组比较[子宫质量(0.45±0.08)g、子宫质量/体质量3.45±0.25],差异均有统计学意义(均P<0.01)。显微镜下观察大鼠子宫内膜,去卵巢组子宫内膜腺体小而稀少,使用不同剂量GST的低、中及高剂量组子宫内膜仍与去卵巢组一样,分布不均匀,腺体内衬上皮由立方或扁平细胞组成,明显富于细胞。结论不同剂量GST均不会促进去势大鼠子宫质量增加及子宫内膜增生,在临床应用可能是安全的。Objective To investigate the effect of different doses of genistein （GST）on uterus and endometrium in o-variectomized rats. Methods Forty female SD rats received ovariectomy and 10 rats received sham operation （normal control）. The ovariectomized rats were further divided into 4 groups （10 in each）：rats in low- dose, middle- dose and high- dose groups were fed with 25mg/kg＆#183;d, 50mg/kg＆#183;d and 100mg/kg＆#183;d GST, respectively; rats in overiectomized control group were fed with same volume of DMSO as those in normal control group. After 4 weeks, the animals were sacrificed, and uterus specimens were obtained. The ratio of weights of the uterine over body was calculated and histopathological examinations on the uterus were performed. Results After 4 weeks, body weights were increased in al groups, and there was no significant difference（P〉0.05） among 5 groups. The weights of uterus in low, middle, high GST groups and overiectomized control group were 0.18±0.03g, 0.16±0.04g, 0.17±0.05g and 0.15±0.05g, respectively （P〉0.05）, and the uterine/body weight ratio was 0.64±0.05, 0.53± 0.08, 0.58±0.03 and 0.48±0.08, respectively （P〉0.05）. There were significant differences in uterus weight and uterus/body weight ratio between GST groups and normal control group （P〈0.01）. The histopathology showed that uterus from ovariec-tomized group and GST groups had smal er endometrial glands than those from normal rats, and there was uneven distribution of glands and the glands were lined by cuboidal or squamous epithelial cells. Conclusion Different doses of GST have no sig-nificant effect on uterus enlarging and endometrial hyperplasia in ovariectomized rats, suggesting that GST administration might be safe in clinical application.

关 键 词：植物雌激素 三羟异黄酮 子宫 子宫内膜 安全性 

分 类 号：R711.75[医药卫生—妇产科学]