香砂六君子汤对脾气虚证大鼠胃Cajal间质细胞、缝隙连接损伤的修复作用研究  

Repair of Xiangsha Liujunzi Decoction on Damage of Interstitial Cells of Cajal and Gap Junction in the Gastric Muscular Layer of Rats of Pi-Qi Deficiency Syndrome:an Experiment Study

作　　者：李冉[1] 齐清会[1] 谢明征[1] 丁伯龙[1] 张盛林[1] 

机构地区：[1]大连医科大学附属第一医院中西医结合研究院,大连116011

出　　处：《中国中西医结合杂志》2014年第10期1216-1219,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173454)

摘　　要：目的探讨脾气虚证(Pi qi deficiency syndrome,PQDS)大鼠胃Cajal间质细胞(interstitial cells of cajal,ICC)、缝隙连接(gap junction,GJ)损伤和香砂六君子汤的修复作用。方法选择30只健康Wistar大鼠,采用苦寒泻下法建立脾气虚证模型。造模成功后分为脾气虚组(模型组)和香砂六君子汤治疗组(治疗组),每组各15只。另选择15只健康Wistar大鼠作为健康组。治疗组灌胃香砂六君子汤(2 mL/100 g),14天;健康组和模型组按照治疗组方法予等量生理盐水灌胃14天。分别于造模前、干预前及干预后取各组胃肌层组织,采用透射电镜观察ICC和GJ的超微结构改变;免疫组织化学法检测连接蛋白43(Cx43)的数量和分布。结果透射电镜显示:与健康组比较,模型组大鼠ICC和GJ明显损伤;经治疗组干预后ICC和GJ有明显修复趋势。与本组造模前比较,模型组干预前后胃肌层Cx43 IOD值均降低(P<0.05)。与本组干预前比较,治疗组干预后Cx43 IOD值升高(P<0.05)。与健康组比较,模型组干预前后胃肌层Cx43 IOD值均降低(P<0.05)。与模型组比较,治疗组干预后Cx43 IOD值升高(P<0.05)。结论脾气虚证大鼠胃肌层中ICC和GJ超微结构破坏,香砂六君子汤可修复脾气虚证状态下ICC和Cx43的损伤。Objective To explore the repair of Xiangsha Liujunzi Decoction （XSLJZD） on interstitial cells of Cajal （ICC） and gap junction （GJ） in the gastric muscular layer of rats of Pi-qi deficiency syndrome （ PQDS）. Methods PQDS was established using purgative method with bitter and cold drugs in30 healthy Wistar rats. After successful modeling they were randomly divided into the treatment group and the model group, 15 in each group. Another 15 healthy Wistar rats were recruited as the healthy control group. Rats in the treatment group were gastric administered with XSLJZD at 2 mL/100 g body weight, once daily for 14 successive days. Equal volume of normal saline was gastrically administered to those in the healthy control group and the model group. The gastric muscle tissues were taken out before modeling, before intervention, and after intervention, respectively. Ultrastructural changes of ICC and GJ were observed using transmission electron microscope （TEM）. The number and distribution of Connexin43 （Cx43） were detected using immunohistochemistry. Results Results of TEM indicated that compared with the healthy control group, both ICC and GJ in the model group showed obvious injury. ICC and GJ were apparently repaired after intervention in the treatment group. Compared with the same group before modeling, the integrated optical density （IOD） of the Cx43 expression significantly decreased in the model group before and after intervention （P 〈0.05）. Compared with before intervention, the IOD of the Cx43expression significantly increased in the treatment group （P 〈0.05）. Compared with the healthy control group, the IOD of the Cx43 expression significantly decreased in the model group before and after inter- vention （P 〈 0.05）. Compared with the model group, the IOD of the Cx43 expression significantly increased in the treatment group （P 〈 0.05）. Conclusions Ultrastructures of ICC and GJ in the gastric muscular layer of rats of PQDS were obviously damaged. XSLJZD could repa

关 键 词：香砂六君子汤 CAJAL间质细胞 缝隙连接 连接蛋白43 

分 类 号：R285.5[医药卫生—中药学]