不同根吸收阶段乳牙牙周膜干细胞生物学特性研究  

Research about biological properties of PDLSCs during physiological root resorption in deciduous tooth

作　　者：王清超 李蓓 张钰 尚临娟 邬礼政 金岩 王小竞 

机构地区：[1]第四军医大学口腔医院儿童口腔科.军事口腔医学国家重点实验室 [2]第四军医大学口腔医院组织工程中心.军事口腔医学国家重点实验室,陕西西安710032

出　　处：《临床口腔医学杂志》2014年第10期589-593,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81170964);(81020108019);(31030033);973项目(2010CB944800)

摘　　要：目的:比较生理性根吸收不同阶段的乳牙牙周膜干细胞(PDLSCs)的生物学特性,检测其对破骨细胞形成和凋亡相关分子的表达情况,为解释生理性根吸收的调控机制提供实验依据。方法:通过分离、培养处于不同生理性根吸收时期的乳牙PDLSCs(未吸收、中度吸收、重度吸收)与恒牙PDLSCs;采用流式细胞术、MTT实验比较各组PDLSCs的免疫表型、细胞增殖能力;采用成骨、成脂诱导与茜素红染色、油红O染色及Real-time PCR检测各期PDLSCs成骨成脂相关基因表达;采用Western blot检测各组PDLSCs的RANKL、OPG与FasL表达。结果:所分离获得各组乳牙PDLSCs表达间充质干细胞标志分子,与恒牙PDLSCs相比具有较强的增殖能力。其中,重度吸收组乳牙PDLSCs与其它组相比成骨能力最强、成脂能力最差。不同根吸收时期的PDLSCs差异表达RANKL、OPG与FasL蛋白。结论:不同根吸收时期乳牙PDLSCs生物学特性存在差异,RANKL、OPG与FasL的差异表达可能对生理性根吸收过程中破骨细胞的形成与凋亡起到调控作用。Objective： To compare the biological properties of PDLSCs from deciduous tooth with different stages of physiological root resorption and to detect their expression of key factors related to the formation and apoptosis of osteoclast. Method： PDLSCs were isolated and cultured from deciduous tooth with different degrees of root resorption and permanent tooth. The study focused on their immune phenotype, proliferation and mutidifferentiation properties by flow cytometry, MTr assay and osteogenic / adipogenic induction respectively. Further as assayed by Western blot, the study examined the ex- pression of RANKL, OPG and FasL, which are key factors to osteoclast status, of PDLSCs from each group. Result. PDLSCs from all these groups expressed mesenchymal stem cell surface markers and had strong proliferation ability. PDLSCs from the severely resorpted tooth had the strongest osteogenic differentiation and the weakest adipogenesis differentiation potential. The expression level of RANKL, OPG and FasL protein of these PDLSCs were different. Coulusion： Biological properties of PDLSCs from different stages of physiological root resorption were different. The different expression pattern of RANKL, OPG and FasL may contribute to the formation and apoptosis of osteoclast in the proscess of physiological root resorption in decid- uous tooth.

关 键 词：生理性根吸收 牙周膜干细胞 生物学特性 破骨细胞 

分 类 号：R813.1[医药卫生—放射医学]