山羊FAM134B基因克隆、序列分析及其mRNA表达与肌内脂肪含量的相关性  

Cloning, Sequence Analysis of FAM134B and Correlation Analysis Between Its mRNA Expression and Intramuscular Fat Content in Goat(Capra hirus)

作　　者：朱武政[1] 江明锋[1] 唐璐[1] 王永[1] 

机构地区：[1]西南民族大学生命科学与技术学院/青藏高原动物遗传资源保护与利用四川省重点实验室,成都610041

出　　处：《农业生物技术学报》2014年第10期1277-1285,共9页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划(No.2012BAD13B06);四川省科技创新产业链示范工程重大项目(No.2011NZ0003);四川省"十二五"畜禽育种攻关项目(No.2011NZ0099-36);西南民族大学研究生"创新型科研项目"(No.CX2014SZ74)

摘　　要：FAM134B基因对肌内脂肪沉积有重要的调控作用,为了进一步了解FAM134B基因的结构与功能及其对山羊(Capra hirus)肉质的影响,本研究以川中黑山羊为实验动物,采用RT-PCR技术克隆了山羊FAM134B基因,并对核苷酸序列和氨基酸序列进行生物信息学分析,运用实时荧光定量PCR技术检测了FAM134B mRNA的表达情况,对肌肉组织中FAM134B mRNA表达与肌内脂肪(intramuscular fat,IMF)含量进行了关联分析。结果表明,山羊FAM134B基因(GenBank登录号:KF684947.1)cDNA全长1 071 bp,编码356个氨基酸,预测蛋白序列含有4个N-连接的糖基化修饰位点,20多个磷酸化位点;系统进化树分析显示,山羊FAM134B核苷酸序列与牛(Bos taurus)的相似性最高;荧光定量PCR分析表明,FAM134B在山羊的心、肝、脾、肺、肾、背最长肌和腿肌中均有表达,其中在肝脏的表达量最高,脾的表达量最低;相关分析显示:山羊背最长肌、腿肌中FAM134B mRNA表达与肌内脂肪含量呈显著正相关。研究结果显示,FAM134B基因可能对山羊肌内脂肪沉积起着重要的调控作用。本研究为进一步了解FAM134B基因的功能及其在反刍动物IMF代谢中的作用和营养调控机制提供一定基础资料。FAM134B plays a critical role in regulation of intramuscular fat（IMF deposition.For a further understanding of the structure and function of FAM134B gene,as well as its impact on mutton （Capra hirus）quality,RT-PCR technique was adopted to amplify the FAM134B gene,during which Chuanzhong black goat was used as the experimental animal.The nucleotide and amino acid sequence was analyzed using bioinformatics tools.The expression levels of FAM134B in different tissues were detected by fluorogenic quantitative PCR.Additionally,correlation analysis between expression of FAM134B mRNA and IMF content in muscles was done.The results showed that the full length of goat FAM134B gene（GenBank accession No.KF684947.1） was 1 071 bp,and coding 356 amino acid sequence.Four N-glycosylation sites were predicted in the translated FAM134B protein,as well as more than 20 phosphorylation sites.A phylogenic tree analyse revealed that the nucleotide sequence of goat shared the highest identity with the cattle （Bos taurus） FAM134B.Fluorescence quantitative PCR analyse indicated that FAM134B expressed in heart,liver,spleen,lung,kidney,longissimus dorsi muscle and leg muscle,with the highest expression level in liver and the lowest expression level in spleen.Correlation analysis demonstrated that strong positive correlations were observed between expression of FAM134B mRNA and IMF content in longissimus dorsi and crureus.These results suggested that FAM134B gene might play an important role in regulation of IMF deposition in goat.This research provides a basic data for a further understanding of the function of FAM134B gene,and the role during the process of IMF metabolism as well as its mechanism of nutritional regulation in ruminant.

关 键 词：山羊 FAM134B 克隆 MRNA 肌内脂肪(IMF) 

分 类 号：S828.1[农业科学—畜牧学]