单纯性胰腺创伤后大鼠胰腺磷脂酶A2活性及其亚型表达特点  

Characteristics of PLA2 activity and expression of PLA2 subtypes in rat pancreas after simple pancreatic trauma

作　　者：陈光宇[1] 戴睿武[1] 汤礼军[1] 田伏洲[1] 文艺[1] 罗皓[1] 邓超[1] 曹均强 

机构地区：[1]成都军区总医院全军普通外科中心,成都610083

出　　处：《解放军医学杂志》2014年第10期776-781,共6页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81001695);四川省科学事业计划基金(2013JY0046);成都军区总医院院管课题(2013YG-B065)~~

摘　　要：目的构建大鼠单纯性胰腺创伤模型并观察大鼠血清与胰腺组织中磷脂酶A2(PLA2)活性及其亚型表达特点。方法将120只Wistar大鼠按1:2随机分为对照组及撞击组,并分别分为6h、24h、72h、7d 4个亚组,对照组每个时间点10只,撞击组每个时间点20只。各撞击组采用BIM-Ⅲ生物撞击机构建胰腺创伤大鼠模型;对照组仅行开腹手术,翻动胰腺数次后关腹。检测各组大鼠血清与胰腺组织PLA2活性及PLA2各亚型在胰腺组织中的表达与分布特点。结果撞击组大鼠血清与胰腺组织中PLA2活性在创伤后6h开始升高,血清PLA2于72h达高峰,胰腺组织PLA2于24h达高峰,7d时血清与胰腺组织PLA2活性仍高于对照组(P<0.05)。胰腺组织中分泌型磷脂酶A2(sPLA2)在RNA水平及蛋白水平表达均高于胞质型磷脂酶A2(cPLA2)与钙非依赖型磷脂酶A2(iPLA2)。结论胰腺创伤后,血清与胰腺组织PLA2活性均显著增高,PLA2各亚型中,sPLA2在胰腺表达增强最为显著。胰腺创伤后6h^7d均可检出血PLA2活性,有助于胰腺创伤的辅助诊断。Objective To study the characteristics of phospholipase A2（PLA2） activity and expression of PLA2 subtypes in rat serum and pancreas tissue with an established simple pancreatic trauma model in rats. Methods One hundred and twenty Wistar rats were divided into two groups in a one-to-two ratio： control group and impact group. The two groups were subsequently divided into four subgroups at 6h, 24 h, 72 h, 7d time point. For the animals in the impact groups, the pancreas was injured by a BIM-Ⅲ biotical impact machine, and the animals in control group were sham injured. The total catalytic activity of PLA2 in serum and pancreatic tissue, the expressions of mRNA and protein and immunohistochemical localization of secretory PLA2（sPLA2）, cytosolic PLA2（cPLA2） and calcium independent PLA2（iPLA2） in pancreatic tissue were determined after the corresponding pretreatments in each group. Results The total catalytic activity of PLA2 in serum and pancreatic tissue of impact groups increased at 6h after injury, and the PLA2 activity reached peak value at 24 h in pancreatic tissue and at 72 h in serum after trauma, and at 7d in serum and pancreatic tissue were still higher than that in control group at the same time（P0.05）. The results of RT-PCR, Western blotting and immunohistochemistry indicated that the expression of sPLA2 in pancreatic tissue was higher than that of cPLA2 and iPLA2. Conclusions PLA2 activity can be upregulated in serum and pancreas after pancreatic trauma. The expression of sPLA2 is increased most significantly in pancreatic tissue after pancreatic trauma. PLA2 activity may be detected in serum 6h-7d after pancreatic trauma, and is helpful for auxiliary diagnosis of simple pancreatic trauma.

关 键 词：胰腺创伤 动物模型 SPLA2 CPLA2 iPLA2 

分 类 号：R641[医药卫生—外科学]