秦皮乙素对BALB/c裸鼠体内胶质瘤生长的影响  

The Effect of Esculetin on Glioma Growth in BALB/c Nude:An in Vivo Study

作　　者：曹舒雯[1] 胡杨[1] 朱小南[1] 陈汝筑[1] 汪雪兰[1] 

机构地区：[1]中山大学中山医学院药理学教研室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2014年第5期672-679,667,共8页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：美国中华医学基金会CMB资助项目(98-677)

摘　　要：【目的】探讨秦皮乙素(Esc)的体内抗胶质瘤活性,为秦皮乙素进一步研究开发为抗胶质瘤药物提供实验依据。【方法】采用C6细胞前肢腋下皮下注射BALB/c裸鼠种瘤构建荷瘤鼠模型;分为4组:模型组、50 mg/kg秦皮乙素组、100mg/kg秦皮乙素组和阳性对照组;采用瘤体积、相对肿瘤增殖率、瘤质量、肿瘤生长抑制率和体质量5个指标评价秦皮乙素的体内抑瘤效果;采用HE染色鉴定瘤体。【结果】与模型组相比,秦皮乙素可显著抑制雄性荷瘤鼠的瘤体积约10倍,瘤质量约8倍,对其体质量无显著影响,与阳性对照药尼莫司汀(ACNU)相似;对雌性荷瘤鼠瘤体的生长无显著抑制作用。【结论】秦皮乙素在雄性荷瘤鼠体内具有显著抗胶质瘤活性,为秦皮乙素进一步开发为抗胶质瘤药物提供了实验依据。[Objective] This study aimed to investigate the in vivo anti-glioma activity of Esculetin （Esc） to provide experimental evidences for further study of Esculetin as a potential anti-glioma drug.[Method] Rat glioma C6 cell was subcutaneously injected into BALB/c nude mouse to set up the tumor-bearing mouse model.Four groups were taken in this experiment：model group,50 mg/kg Esculetin group,100 mg/kg Esculetin group and positive control group.Tumor volume,relative tumor proliferation rate,tumor weight,inhibition rate and body weight were measured to evaluate the in vivo anti-glioma activity of Esculetin.HE staining was used to identify the tumor tissue.[Results] Comparing with the model group,Esculetin significantly inhibited tumor volume to 1/10,and tumor weight to 1/8 of the model group in male tumor-bearing mouse,similar to the positive control drug Nimusting （ACNU）,while showed no significant anti-glioma activity in female tumor-bearing mouse.[Conclusion] Esculetin exhibits significant anti-glioma activity on male tumor-bearing mouse,thus provides experimental evidences for further study of Esculetin as a potential anti-glioma drug.

关 键 词：秦皮乙素 胶质瘤 BALB/C裸鼠 抑瘤效果 

分 类 号：R979.1[医药卫生—药品]