造血系统常用Cre转基因小鼠及其应用  

Commonly Used Cre Transgenic Mice and Their Applications in Hematopoietic System——Review

作　　者：彭路芸 程涛 袁卫平 

机构地区：[1]中国医学科学院、北京协和医学院血液学研究所、血液病医院,实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2014年第5期1442-1447,共6页Journal of Experimental Hematology

基　　金：科技部重大基础研究项目(2011CB964801,2012CB966604,2013CB966902);国家自然科学基金项目(81090410,81130074,81070390)

摘　　要：Cre-lox重组系统包括Cre重组酶和lox位点两个要素。Cre酶能够特异地识别并催化lox位点间的片段进行重组,lox位点的方向和位置则决定Cre酶的功能效应。小鼠造血系统由多系各个发育阶段的造血细胞组成。它们是由骨髓内的造血干细胞(Hematopoietic stem cell,HSC)分化发育而来,而造血细胞的增殖发育是在骨髓微环境中进行的。目前有利用Cre-lox重组系统建立的多种转基因小鼠运用于造血系统的研究中,本文将讨论常用于造血系统的Cre转基因小鼠以及它们在造血系统研究中的应用。Cre-lox recombination system consists of two elements：Cre recombinase enzyme and lox sites.Cre recombinase can recombine the lox site sequences by specifically detecting and cutting them.The direction and position of lox sites determine the functional effects of Cre enzyme such as deletion,inversion or chromosomal translocation.The hematopoietic system of mouse consists of multi-lineages and various developmental stage hematopoietic cells that are differentiated from hematopoietic stem cells （hematopoietic stem cells,HSC）.The hematopoietic stem cells are maintained in the bone marrow microenvironment （niche）.Currently,a variety of floxed conditional-knockout mice,recognized by Cre-lox recombination system,are used for the study of the hematopoietic system.This review summarizes the commonly used Cre transgenic mice and their applications in the study of hematopoietic system.

关 键 词：转基因小鼠 CRE-LOX 造血系统 

分 类 号：R332.2[医药卫生—人体生理学]