外周血单个核细胞DNA提取方法的研究进展  

Research Advances on DNA Extraction Methods from Peripheral Blood Mononuclear Cells——Review

作　　者：王晓英[1] 余晨曦[2] 

机构地区：[1]东南大学公共卫生学院营养与食品卫生学系,江苏南京210009 [2]东南大学医学院临床医学学系,江苏南京210009

出　　处：《中国实验血液学杂志》2014年第5期1495-1498,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(81302472);教育部博士点基金(20110092120055);大学生创新性实验项目(S2013114;T13422001)

摘　　要：DNA的提取是分子生物学研究的基础技术,提取的DNA的纯度及结构完整性是进行基因工程各项研究所必需的条件。外周血作为一种常用的临床检测材料,如何从外周血单个核细胞中快速、高效的分离提取基因组DNA,对于临床血液检验与分析非常重要。目前,DNA的抽提方法有很多,如酚-氯仿法、盐析法、离心吸附柱法等人工方法,以及磁珠法等半自动方法及试剂盒法。本文就从外周血血液中提取DNA方法的原理、具体操作步骤及方法评估等给予简要的综述。DNA extraction is a basic technology of molecular biology. The purity and the integrality of DNA structure are necessary for different experiments of gene engineering. As commonly used materials in the clinical detection, the fast, efficient isolation and extraction of genomic DNA from peripheral blood mononuclear cells is very important for the inspection and analysis of clinical blood. At present, there are many methods for extracting DNA, such as phenol-chloroform method, salting out method, centrifugal adsorption column chromatography method （artificial methods）, magnetic beads （ semi-automatic method） and DNA extraction kit. In this article, a brief review of the principle for existing DNA blood extraction method, the specific steps and the assessment of the specific methods briefly are summarized.

关 键 词：外周血 单个核细胞 DNA提取 

分 类 号：R446.1[医药卫生—诊断学]