万古霉素阳离子脂质体复合纳米羟基磷灰石/壳聚糖/魔芋葡苷聚糖支架对金黄色葡萄球菌生物膜体外抑制作用  

Nano-hydroxyapatite /chitosan /konjac glucomannan scaffolds loaded with cationic liposomal vancomycin: preparation and activity against Staphylococcus aureus biofilms in vitro experiments

作　　者：马涛[1] 尚北城[1] 陈庆华[2] 徐帆[1] 周田华[1] 徐永清[1] 

机构地区：[1]成都军区昆明总医院骨科,云南昆明650032 [2]昆明理工大学材料科学与工程学院,云南昆明650032

出　　处：《临床骨科杂志》2014年第5期595-600,共6页Journal of Clinical Orthopaedics

基　　金：国家自然科学基金(编号:81171734);云南省科技厅重点新产品开发项目(编号:2012BC007)

摘　　要：目的研究万古霉素阳离子脂质体(CLVs)复合纳米羟基磷灰石/壳聚糖/魔芋葡苷聚糖支架对金黄色葡萄球菌生物膜的抑制作用。方法采用微量肉汤稀释法测定药物对金黄色葡萄球菌的最小抑菌浓度,采用再生实验方法研究支架释放的CLVs对金黄色葡萄球菌生物膜的敏感性。结果万古霉素和CLVs对金黄色葡萄球菌的最小抑菌浓度分别为1.0 mg/L和0.6 mg/L;相同浓度的万古霉素脂质体复合支架对细菌生物膜的作用在低浓度和短时间的接触较游离万古霉素支架更有效。结论 CLVs复合纳米羟基磷灰石/壳聚糖/魔芋葡苷聚糖支架可以作为新的载药系统,在临床治疗生物膜感染方面可起到重要作用。Objective To prepare the cationic liposomal vancomycin （ CLVs） loaded nano-hydroxyapatite/chitosan/konjac glucomannan scaffolds and elucidate the activity against Staphylococcus aureus biofilms. Methods The mini-mal inhibitory concentration（ MIC） of samples to Staphylococcus aureus was determined by a broth microdilution assay. The biofilm susceptibility to liposomal vancomycin was assessed using a regrowth assay. Results The MIC of free vancomycin and CLVs against Staphylococcus aureus were 1. 0 mg/L and 0. 6mg/L, respectively. Liposomal vancomy-cin loaded with scaffolds was more effective at inhibiting the formation of Staphylococcus aureus biofilms than the free drug-loaded scaffold at equivalent vancomycin doses, especially at low concentrations and short exposure times. Con-clusions The n-HA/CS/KGM loaded with CLVs may be a new drug delivery system of antibiotics for treatment of biofilm infections.

关 键 词：纳米羟基磷灰石/壳聚糖/魔芋葡苷聚糖支架 阳离子脂质体 万古霉素 生物膜 

分 类 号：R978.1[医药卫生—药品] R318.021[医药卫生—药学]