附睾蛋白酶抑制剂(EPPIN)基因在男性不育患者精子中的表达情况分析  

Expression of EPPIN Gene in Human Sperm and Its Significance

作　　者：于艳[1] 张琪瑶[1] 杨伟娜[1] 孔风云[1] 张斌[1] 菅福琴[1] 郑颖[1] 

机构地区：[1]山东中医药大学第二附属医院生殖科,济南250001

出　　处：《生殖与避孕》2014年第10期795-799,823,共6页Reproduction and Contraception

基　　金：国家青年基金(81102653);山东省自然科学基金(ZR2012HL21;ZR2011HM013)项目

摘　　要：目的:探讨附睾蛋白酶抑制剂(epididymal protease inhibitor,EPPIN)基因在男性不育患者精子中的表达情况及其对男性生育力的影响。方法:收集少弱精子症组(n=8)、弱精子症组(n=12)及对照组(n=19)的精液标本,分别采用Western blotting、半定量RT-PCR和实时荧光定量PCR(qRT-PCR)方法检测各组精子中EPPIN的表达,并计算其相对表达量及与精液质量的相关性。结果:EPPIN基因在不同组精子中均有表达,在对照组、弱精子症组和少弱精子症组的EPPIN mRNA的相对表达量分别为0.09±0.02、0.29±0.08和1.00±0.18,各组间比较差异有显著统计学意义(P<0.01)。EPPIN蛋白的相对表达量与mRNA类似。EPPIN mRNA表达量与精子浓度、前向运动精子(progressive motility,PR)百分率和活力呈显著负相关。结论:EPPIN可能与人类精子数量及活力有关,推测可能参与少弱精子症的发病机制。Objective： To investigate the expression of the epididymal protease inhibitor（EPPIN） gene in human sperm and its clinical significance in male fertility. Methods： A total of 39 semen samples were collected and divided into 3 groups： control group（n=19）, asthenozoospermia group（n=12） and oligoasthenozoospermia group（n=8）. The EPPIN mRNA expression and its protein expression were detected by Western blotting and qRTPCR. The correlation between EPPIN expression level and sperm quality was detected. Results： The EPPIN gene was expressed in all the three groups; EPPIN mRNA relative expressions in the control, asthenozoospermia and oligoasthenozoospermia groups were 0.09±0.02, 0.29±0.08 and 1.00±0.18, respectively, there was a significant difference among the three groups. EPPIN protein relative expression was similar to those of EPPIN mRNA in the three groups. There was a negative correlation between EPPIN mRNA expression and sperm concentration, progressive motility and sperm motility. Conclusion： EPPIN expression is correlated with sperm quantity and viability. This finding suggests that EPPIN may be involved in the pathogenesis of oligoasthenozoospermia patients.

关 键 词：附睾蛋白酶抑制剂(EPPIN) 男性不育 少弱精子症 精子 

分 类 号：R698.2[医药卫生—泌尿科学]