脂肪干细胞治疗放射性肠炎的实验研究  

Therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis

作　　者：常鹏宇[1] 崔爽[1] 罗景华[1] 曲超[1] 姜新[1] 曲雅勤[1] 董丽华[1] 

机构地区：[1]吉林大学白求恩第一医院放疗科,长春130021

出　　处：《中华放射医学与防护杂志》2014年第10期729-734,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（81372929）

摘　　要：目的 评价脂肪干细胞对放射性肠炎的治疗作用.方法 选用成年雄性SD大鼠,共52只.随机数字表法选取46只给予全腹15 Gy X射线照射制作放射性肠炎动物模型.造模结束后2h,随机数字表法从受照大鼠中选取23只受照大鼠给予P6代青年女性脂肪干细胞腹腔注射治疗（Ad-MSC治疗组）;取另23只受照大鼠给予双磷酸盐缓冲液（PBS）腹腔注射治疗（PBS溶剂对照组）;剩余6只未照射大鼠作为健康对照组.造模后首个10d内,分次抽取受照大鼠外周血,ELISA法检测血清中白介素-10（IL-10）的含量;分离受照大鼠胸腺细胞及外周血单个核细胞（PBMC）,流式细胞术检测CD4/CD25/Foxp（3）阳性调节性T细胞的百分含量;获取受照小肠组织,HE染色分析炎症细胞浸润程度,Masson三色染色分析胶原成分的沉积程度,髓系过氧化物酶（MPO）免疫组织化学染色分析浸润的炎症细胞类型.Kaplan-Meier方法对受照大鼠进行生存分析.结果 在照射后30 d内,与PBS腹腔注射治疗的大鼠相比,接受脂肪干细胞治疗的大鼠的生存时间显著延长（t=4.53,P ＜0.05）.另外,脂肪干细胞能够上调受照大鼠外周血中IL-10的含量（7 d：t=13.93,P＜0.05）;上调外周血（3.5 d：t =7.72,7 d：t =11.11,10 d：t=6.99,P＜0.05）和胸腺（7 d：t =16.17,10 d：t=12.12,P＜0.05） CD4/CD25/Foxp（3）阳性调节性T细胞的百分比;进而减轻炎症细胞在受照小肠中的浸润及胶原的沉积.结论 脂肪干细胞对放射性肠炎具有治疗作用.Objective To evaluate the therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis.Methods A total of 52 male Sprague-Dawley rats were used in the present study.Herein,46 rats were randomly selected and irradiated with a dose of 15 Gy at their abdomens.Two hours post-irradiation,23 rats were randomly selected and infused intraperitoneally with adipose-derived mesenchymal stem cells in passage 6 from young-female donor.The other 23 rats were intraperitoneally infused with PBS.The rest 6 rats were set as normal control.During the first 10 days post-irradiation,peripheral blood-samples from irradiated rats were harvested for testing the levels of IL-10 in serum using ELISA assay.Additionally,after isolating the thymic cells and peripheral blood mononuclear cells,the percentages of CD4/CD25/Foxp（3）-positive regulatory T cells in thymus and peripheral blood were tested by flow-cytometry.Finally,infiltration of inflammatory cells and deposition of collagens within irradiated small intestine were analyzed by H＆E staining and Masson Trichrome staining,respectively.Based on the MPO-immunohistochemistry staining,the type of infiltrated cells was identified.The Kaplan-Meier method was used for analyzing the survival rate of irradiated rats.Results During a period of 30 days post-irradiation,the irradiated rats receiving adipose-derived mesenchymal stem cells survived longer than those receiving PBS （t =4.53,P 〈 0.05）.Compared to the irradiated rats with PBS-treatment,adipose-derived mesenchymal stem cells could elevate the level of IL-10 in serum （7 d：t =13.93,P 〈 0.05） and increase the percentages of CD4/CD25/Foxp（3）-positive regulatory T cells in both peripheral blood （3.5 d：t =7.72,7 d：t=11.11,10 d：t =6.99,P 〈0.05） and thymus （7 d：t =16.17,10 d：t =12.12,P〈 0.05）.Moreover,infiltration of inflammatory cells and deposition of collagens within irradiated small intestine were mitigated by adipose-derived mesenchymal stem cells.Conclusions Adipose-derived

关 键 词：脂肪干细胞 放射性肠炎 细胞治疗 

分 类 号：R818[医药卫生—放射医学]