Simultaneous determination of three curcuminoids in Curcuma longa L.by high performance liquid chromatography coupled with electrochemical detection  

作　　者：Yuling Long Wenpeng Zhang Fang Wang Zilin Chen 

机构地区：[1]Key Laboratory of Combinatorial Biosynthesis and Drug Discovery,Wuhan University,Ministry of Education [2]Wuhan University School of Pharmaceutical Sciences

出　　处：《Journal of Pharmaceutical Analysis》2014年第5期325-330,共6页药物分析学报（英文版）

基　　金：supported by the National Scientific Foundation of China (Grant nos.21375101,90817103,and 30973672);Doctroral Fund of Ministry of Education of China (No.20110141110024);Innovation Seed Fund and Translational Medical Research Fund of Wuhan University School of Medicine

摘　　要：A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4-H3PO4 （pH 5.0） （50：50, v/v） was used. Good linearity was obtained in the range of 0.208-41.6, 0.197-39.4, and 0.227-114μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10 ？ 8 M, which was lower than that by UV detection. The relative standard deviations （RSDs） ranged from 1.06%to 1.88%for intra-day precision and from 4.30%to 5.79%for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3%to 111%were obtained.A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4-H3PO4 （pH 5.0） （50：50, v/v） was used. Good linearity was obtained in the range of 0.208-41.6, 0.197-39.4, and 0.227-114μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10 ？ 8 M, which was lower than that by UV detection. The relative standard deviations （RSDs） ranged from 1.06%to 1.88%for intra-day precision and from 4.30%to 5.79%for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3%to 111%were obtained.

关 键 词：CURCUMIN High performance liquidchromatography Electrochemical detec- tion Curcuma longa L. 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]