猪microRNA组学研究进展  

Advances in porcine miRNAome

作　　者：冉茂良[1,2] 陈斌[1,2] 尹杰[3,4] 杨岸奇[1,2] 李智[1,2] 蒋明[1,2] 

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]畜禽遗传改良湖南省重点实验室,长沙410128 [3]中国科学院亚热带农业生态研究所,长沙410125 [4]中国科学院研究生院,北京100049

出　　处：《遗传》2014年第10期974-984,共11页Hereditas（Beijing)

基　　金：国家现代农业产业技术体系建设专项资金(CARS-36)资助

摘　　要：MicroRNA(miRNA)是一类长约22 nt的非编码小RNA,广泛存在于各种生物中,调节生物体生长、发育和凋亡等过程。研究表明,miRNA在猪肌肉、脂肪、生殖系统以及免疫系统等的发育过程中发挥着重要的调控作用。此外,高通量的新一代测序技术在猪miRNA的挖掘和差异表达研究中发挥着巨大的作用。文章综述了高通量的新一代测序技术在挖掘猪miRNA中的应用以及一些miRNA在猪脂肪代谢、肌肉发育、卵母细胞成熟和B、T淋巴细胞发育中的调控作用,旨在为猪miRNA的研究提供参考,为利用miRNA调控和改善猪肉品质、生长性能、繁殖性能以及免疫机能提供理论基础和研究思路。MicroRNA （miRNA）, a class of non-coding RNA （about 22 nt）, widely exists in different organisms and plays an important role in growth, development, and apoptosis. Recently, many studies have shown that miRNAs serve as a regulatory mechanism in mediating the development of pig muscle, fat, reproductive and immunity traits. The next-generation of high-throughput sequencing technology plays a critical role on finding new miRNAs and identifying their different expressions in pig. In this review, we summarize the application of the next-generation of high-throughput sequencing technology in the study of pig miRNAs as well as the regulatory roles of several impor-tant miRNAs in fat metabolism, muscle development, oocyte maturation and development of B cells and T cells. This review will provide insight into the research on pig miRNAs, and some ideas on how to improve pork quality, growth, reproductive and immunity performance.

关 键 词：猪 MICRORNA 脂肪代谢 肌肉发育 卵母细胞成熟 免疫 

分 类 号：S828[农业科学—畜牧学]