电针对坐骨神经慢性缩窄损伤大鼠脊髓α-氨基-3-羧基-5-甲基异恶唑-4-丙酸受体表达的影响  

Effect of Electroacupuncture Intervention on Spinal AMPA-receptor Expression in Rats with Neuropathic Pain

作　　者：闫丽萍[1] 侯保权 吴辛甜 王玲玲[1] 马骋[1] 

机构地区：[1]南京中医药大学江苏省针灸学重点实验室,南京210029 [2]南京凯基生物科技发展有限公司,南京210012 [3]常州市中医院针灸科,常州213003

出　　处：《针刺研究》2014年第5期382-386,共5页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81173329);江苏省自然科学基金基础研究计划(No.BK 2008458)

摘　　要：目的:观察电针对坐骨神经慢性缩窄损伤大鼠脊髓相应节段α-氨基-3-羧基-5-甲基异恶唑-4-丙酸(α-amino-3-ydroxy-5-methylisoxazole-4-propionate,AMPA)受体表达的影响,探讨电针干预神经病理性痛的脊髓机制。方法:SD大鼠分为假手术组、模型组和电针组,各20只,采用坐骨神经慢性缩窄损伤法制备神经病理性痛模型。电针组电针大鼠损伤侧"委中"与"环跳"穴,每次30min,每日1次,治疗7d。采用免疫组化法和蛋白印迹法测定脊髓AMPA受体亚基1(GluR 1)的表达,采用反转录-聚合酶链反应(RT-PCR)法测定脊髓GluR 1mRNA的表达。结果:与假手术组比较,模型组脊髓GluR 1免疫组化与蛋白印迹表达量均升高(P<0.05,P<0.01),GluR 1mRNA的表达升高(P<0.05);与模型组比较,电针组脊髓GluR 1免疫组化与蛋白印迹及其mRNA表达均被逆转(P<0.05)。结论:电针能够减轻大鼠神经病理性痛的机制可能与有效地下调脊髓AMPA受体GluR 1的表达有关。Objective To observe the effect of electroacupuncture（EA）intervention on changes of spinal a-amino-3-ydroxy-5-methylisoxazole-4-propionate acid（AMPA）receptor（GluR 1）expression in rats with chronic constrictive injury（CCI）pain,so as to explore its mechanisms underlying improvement of neuropathic pain.Methods Sixty male SD rats were randomly divided into sham-operation,CCI model and EA groups（n=20）.Neuropathic pain model was established by ligature of the right sciatic nerve.EA stimulation（2Hz,1-3mA）was applied to＂Weizhong＂（BL 40）and＂Huantiao＂（GB 30）on the injured limb for 30 min,once a day for 7days beginning from the 11 th day on after CCI.The mechanical and thermal pain thresholds were measured before and after the CCI procedure（baseline）and after EA intervention.The AMPA receptor subunit GluR 1protein and gene expression in L 5-L 6segments of the spinal cord was detected using Western blot（WB）,immunohistochemistry and reverse transcription（RT）-polymerase chain reaction（PCR）,separately.Results As the results of mechanical and thermal pain thresholds in our past study,EA intervention could markedly raise CCI-reduced decreased pain threshold.Compared with the sham-operation group,the expression levels of spinal GluR 1protein and mRNA in the model group were significantly increased（P0.05,P0.01）.Following EA intervention,the expression levels of GluR 1protein and mRNA were remarkably down-regulated in the EA group in comparison with those of the model group（all P0.05）.Conclusion EA intervention can down-regulate CCI-induced increase of AMPA receptor GluR 1expression in the lumbar spinal cord in CCI rats,which may contribute to its effect in alleviating neuropathic pain.

关 键 词：电针 神经病理性痛 腰部脊髓 AMPA受体/GluR 1表达 

分 类 号：R245.97[医药卫生—针灸推拿学]