N-乙酰半胱氨酸对模拟失重肺炎大鼠的保护性研究  

The protective effect of N-acetylcysteine onstreptococcus pneumonia model of simulated weightlessness rats

作　　者：王小辉[1] 王萍[1] 朱敏立[1] 李月越[1] 徐冰心[2] 周金莲[3] 易勇[4] 

机构地区：[1]安徽医科大学解放军第306医院临床学院呼吸与重症医学科,北京100000 [2]安徽医科大学解放军第306医院临床学院特种病科,北京100000 [3]安徽医科大学解放军第306医院临床学院病理科,北京100000 [4]安徽医科大学解放军第306医院临床学院微生物室,北京100000

出　　处：《安徽医科大学学报》2014年第11期1544-1548,共5页Acta Universitatis Medicinalis Anhui

基　　金：全军医学科学技术研究"十二五"重点项目(编号:BWS11J051)

摘　　要：目的探讨在模拟失重(微重力)状态下,N-乙酰半胱氨酸(NAC)对呼吸系统感染肺炎链球菌时的保护作用。方法将32只清洁级Wistar大鼠随机均分为4组:尾吊灌药注菌组(A组)、尾吊灌药未注菌组(B组)、尾吊未灌药注菌组(C组)、尾吊未灌药未注菌组(D组)。从实验第1天开始A、B组灌胃给予NAC 300 mg/kg,C、D组予以等量无菌注射用水灌胃。第2天采用国际通用的持续尾吊法建立模拟失重模型,A、C组于第4天通过气管注入0.4 ml肺炎链球菌稀释液。B、D组注入等量无菌生理盐水。尾吊7 d后处死大鼠取材,测定血常规、中性粒细胞表面CD11b/c、中性粒细胞活性氧浓度、白介素-10(IL-10)、白介素-6(IL-6)含量。病理切片观察肺组织结构变化。结果 C组显微镜下明显可见肺泡大小不等,肺泡间隔增宽增厚,间质内大量中性粒细胞、淋巴细胞浸润,肺泡腔内可见大量泡沫细胞。A组也可见上述病理变化,但较C组减轻,D组次之,B组病变最轻,炎症反应不明显。A、C组白细胞总数、中性粒细胞总数、中性粒细胞百分比高于B、D组,差异有统计学意义(P<0.05,P<0.01)。A组与C组,B组与D组比较差异无统计学意义。各组间CD11b/c、中性粒细胞活性氧浓度、IL-10、IL-6含量差异有统计学意义(P<0.01,P<0.05)。结论 N-乙酰半胱氨酸能够减轻模拟失重状态下大鼠肺炎链球菌肺炎的炎症反应和肺组织破坏,其抗氧化应激作用可能对呼吸系统感染有一定保护作用。Objective To explore the ability of resistance to the infection of Streptococcus pneumoniae of the body during simulated weightlessness, which was set up through tail suspension. Besides this, the protective role of N- acetylcysteine was also observed. Methods The simulated microgravity and infected animal model： Healthy clean male Wistar rats （ n = 32）, weight （270 ± 20） g, randomly divided into 4 groups ： Group A （ tail suspension, in- fection and NAC group）, Group B （tail suspension, NAC and no infection group）, Group C （tail suspension, in- fection and no NAC group） , Group D （tail suspension, no infection and no NAC group）. NAC [ 300 mg（ kg ·d） ] were fed by intragastric administration on the first day, while the same amount of distilled water were fed in control group. On the second day, the models of simulated weightlessness were duplicated by tail suspension meth- od （Each rat was hung by its tail in a cage, and 30 degrees with the horizontal）. The rats were intratracheal in- stilled with 0. 4 ml suspension of streptococcus pneumoniae after tail-suspension for 3 days. At the same time, no- infection groups were intratracheal instilled with 0.4 ml sterile saline. 3 t the end of experiment, the rats were kill- ed. The leukocytes and neutrophils, CD11 b and DCFH-DA in the blood were studied. Results ① The pathologi- cal changes of rat lung ： in Group C, obvious congestion of the lung tissue could be observed. Some of alveolus got fusion, alveolar septum got sick, lung＇ s small veins and capillaries dilated and congestion. Large number of neu- trophils and lymphocytes infiltrated in the interstitial, foam cells flooded in the alveolar space. Some changes like these could be found in Group A, but less severe than Group C. A few of the same changes but less severe could be observed in Group D. ② Compared with Group A and Group C, the percentage of neutrophile was significantly higher than that in Group B and Group D. And the same trend could be observed in leukocyte

关 键 词：失重模拟 大鼠 N-乙酰半胱氨酸 肺炎链球菌 肺部感染 

分 类 号：R332[医药卫生—人体生理学]