间充质干细胞在哮喘大鼠气道炎症及气道重塑中的作用  

The role of mesenchymal stem cells on airway inflammation and airway remodeling in asthma

作　　者：李洁[1] 莫碧文[1] 毛雨红 李劳东 王志霞[1] 牛坤汀 王昌明[1] 曾锦荣[1] 韦江红[1] 黄剑伟[1] 

机构地区：[1]桂林医学院附属医院呼吸内科,桂林541001 [2]衡阳市第一人民医院呼吸内科,衡阳421001 [3]柳州市工人医院呼吸内科,柳州545005

出　　处：《安徽医科大学学报》2014年第11期1549-1552,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81260009);广西自然科学基金重点课题(编号:2012GXNSFDA053020)

摘　　要：目的探讨间充质干细胞(MSCs)在哮喘大鼠慢性气道炎症及气道重塑中的作用。方法将18只SD雌性大鼠随机分为:正常对照组、哮喘4周组和哮喘8周组,每组6只。卵清蛋白(OVA)致敏后,雾化吸入OVA制作哮喘模型。哮喘模型成功后,测定气道压力;通过HE染色、Image-Pro Plus图像分析软件分析大鼠气道平滑肌的嗜酸性粒细胞(ESO)浸润情况,测定支气管管腔的内周长、管壁面积,支气管平滑肌面积、平滑肌细胞核数;采用密度梯度离心法分离外周血单个核细胞,通过流式细胞仪检测各组MSCs的含量,并分析其与哮喘气道炎症及气道重塑的关系。结果哮喘4周组、哮喘8周组大鼠的气道反应性、气道壁EOS计数、支气管管壁面积、支气管平滑肌面积、平滑肌细胞核数目、外周血中MSCs比例均较正常对照组显著增加(P<0.01,P<0.05);哮喘两组间上述指标差异均有统计学意义(P<0.01);Pearson线性相关显示:各组大鼠外周血单个核细胞中MSCs的含量与气道反应性、EOS浸润数、支气管壁面积、支气管平滑肌厚度及支气管平滑肌细胞核数均呈正相关(P<0.01)。结论 MSCs在哮喘状态下含量增加,可能参与了哮喘的慢性气道炎症及气道重塑。Objective To explore the effect of mesenchymal stem cells on chronic airway inflammation and airway remodeling in a rat asthma model. Methods Eighteen SD female rats were randomly divided into 3 groups （n = 6） ： control group, asthmatic 4 weeks group and asthmatic 8 weeks group. The rats were sensitized and inhaled with ovalbumin （OVA） to make asthmatic model. After successfully establishing model of asthma, the airway pressure had to be measured. The methods of HE staining and Image-Pro Plus image analysis software were used to detect the changes of eosinophils granulocytes （ EOS）, the perimeter inner of the bronchial lumen, the wall area, the area of bronchial smooth muscle and the number of smooth muscle cells. Density gradient centrifugation was used to iso- late mesenchymal stem cells in peripheral blood, while the content of mesenchymal stem cells could be detected by the flow cytometry. The Role of mesenchymal stem cells on airway inflammation and airway remodeling in asthma was able to be analyzed from the above data. Results The airway responsiveness, the count of EOS, the area of bronchial wall, the area of bronchial smooth muscle, the number of smooth muscle cells and the proportion of mes- enchymal stem cells in the peripheral blood in the asthmatic 4 weeks group and asthmatic 8 weeks group was signifi- cantly higher than those in the control group （ P 〈 0. 01, P 〈 0. 05 ）. Significant difference between two asthmatic groups was observed inthe above indexes（P 〈0.01 ）. Pearson linear correlation demonstrated that the content of mesenchymal stem cells was significantly positively correlated with the airway responsiveness, the number of EOS, the area of bronchial wall, the area of bronchial smooth muscle and the number of bronchial smooth muscle ceils （P 〈0.01）. Conclusion The mesenchymal stem cells increase in the state of asthma, which may be involved in chronic airway inflammation and airway remodeling of asthma.

关 键 词：间充质干细胞 支气管哮喘 气道炎症 气道重塑 

分 类 号：R562.2[医药卫生—呼吸系统] R322.34[医药卫生—内科学]