白花蛇舌草总黄酮的抗肿瘤作用  

Antitumor effect of total flavones of Oldenlandia diffusa Willd

作　　者：王宇翎[1] 张艳[1] 李前进[1] 林杰[1] 

机构地区：[1]安徽医科大学基础医学院药理学教研室,合肥230032

出　　处：《安徽医科大学学报》2014年第11期1622-1625,共4页Acta Universitatis Medicinalis Anhui

基　　金：高校省级自然科学研究项目(编号:KJ2010B396)

摘　　要：目的研究白花蛇舌草总黄酮(FOD)的体内外抗肿瘤作用。方法建立了小鼠肉瘤S180、EAC腹水型、肝癌HepA实体型移植肿瘤模型,观察FOD(0.5、1.0、5.0、10.0mg/kg)的体内抗肿瘤作用;采用体外试验观察FOD的体外抗肿瘤作用。结果 FOD(5.0、10.0 mg/kg)对S180小鼠肿瘤有明显的抑制作用,FOD(1.0、5.0、10.0 mg/kg)对小鼠HepA实体瘤有明显的抑制作用,FOD对腹水型EAC小鼠无保护作用。FOD半数抑制浓度(IC50)均值分别为MCF-7细胞92.8μg/ml;BEL-7407细胞147.7μg/ml;SMMC-7721细胞147.9μg/ml;BGC-823细胞241.3μg/ml;COLO-205细胞247.1μg/ml;Hep-2细胞284.8μg/ml;A549细胞323.4μg/ml;Hela细胞575.5μg/ml。结论 FOD具有一定的体内外抗肿瘤作用。Objective To investigate the antitumor effects of total flavones of Oldenlandia diffusa Willd （FOD）. Methods Mice sarcoma S180 model, EAC ascites model and liver tumor HepA entity model transplanted tumor model were established to observe the antitumor effect in vivo and in vitro through the experiment. Results In vivo tests, FOD in a dosage of 5 and 10 mg/kg inhibited the growth of S180 in mice with the tumor inhibitory rate （IR） more than 30% ; FOD in a dosage of 1, 5 and 10 mg/kg inhibited the growth of HepA in mice with an IR more than 30% and FOD on EAC in mice with an IR less than 30%. In vitro tests, treatment of MCF-7 cells, BEL-7407 cells, SMMC-7721 cells, BGC-823 cells, COLO-205 cells, Hep-2 cells and A549 cells with FOD, with mean IC50 values were 92. 8 μg/ml, 147.7 μg/ml, 147.9 μg/ml, 241.3 μg/ml, 247. 1 μg/ml, 284.8 μg/ml, 323.4 μg/ ml, 575.5 μg/ml, respectively. Conclusion FOD shows significant antitumor effect experimentally in vitro and in vivo.

关 键 词：白花蛇舌草总黄酮 肿瘤 

分 类 号：R284[医药卫生—中药学] R73[医药卫生—中医学]