硫代硫酸钠在大鼠血管钙化中的干预作用与机制  

Intervention of sodium thiosulfate and mechanisms of vascular calcification in rats

作　　者：王珣[1] 任伟[1] 汪鹏[1] 王科[1] 倪力军[1] 方媛[1] 潘卉萱[1] 

机构地区：[1]安徽医科大学附属省立医院肾内科,合肥230001

出　　处：《安徽医科大学学报》2014年第11期1626-1629,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省卫生厅医学科研课题(编号:2010C040)

摘　　要：目的研究硫代硫酸钠(STS)在大鼠血管钙化中的干预作用与机制。方法用含有750 mg/kg腺嘌呤的颗粒饲料喂养SD大鼠7周制备尿毒症大鼠模型。设正常对照组、尿毒症组、STS组,每组各10只大鼠,包括5只雄鼠和5只雌鼠。用腺嘌呤饲料喂养大鼠7周以后,予大鼠STS每次0.5 g/kg腹腔注射,每周3次,持续5周,并于第12周处死动物。取血测血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平,用ELISA法测定各组大鼠血浆基质Gla蛋白(MGP)、胎球蛋白A(FA)水平。结果与正常对照组比较,尿毒症组大鼠血浆SOD活性降低而MDA水平升高,差异有统计学意义(P<0.05)。STS组大鼠血浆SOD活性降低和MDA水平升高受到一定程度的抑制,差异有统计学意义(P<0.05),尿毒症大鼠血浆MGP、FA水平显著降低,STS组大鼠MGP、FA水平升高,与尿毒症组比较差异有统计学意义(P<0.05)。结论 STS能够治疗并减轻尿毒症大鼠血管钙化的发生。STS可以通过提高尿毒症大鼠血浆MGP和FA水平,从而抑制血管钙化的发生,并且其具有抗氧化特性,能减轻氧化应激损伤,改善血管内皮功能,这也可能是其预防钙化的机制之一。Objective To study the intervention effect and mechanism of sodium thiosulfate （STS） in the vascular calcification of the rat. Methods SD rats were fed with pellet feed for 7 weeks to prepare the uremic rats model, which contained 750 mg adenine per kg. Rats were divided into three groups： normal control group, the uremic rats group and the uremic rats and STS group. Each group had 10 rats, including 5 male rats and 5 female rats . These rats were treated with adenine for 7 weeks,then intraperitoneally injected with STS [0. 5 g/（ kg · w）,Tid for 5 weeks] and killed at last. Blood was used to test serum SOD and MDA level and determine MGP and fetuin A （FA） level. Results Compared with the normal control groups, the plasma SOD activity of uremic rats decreased and the the MDA level increased. The difference was statistically significant （P 〈 0. 05）. The plasma SOD activity of the STS treatment rats group decreased and the increase of MDA level was controlled to some extent. The differ- ence was statistically significant （ P 〈 0. 05 ）. The MGP and FA level of the uremic rats group decreased obviously. The MGP and FA level of the STS treatment rats group increased. The difference between the STS treatment rats group and the uremic rats group was statistically significant （P 〈 0. 05 ）. Conclusion STS can treat and ease the vascular calcification of the uremic rats. STS can restrain vascular calcification by increasing the MGP and FA level of the uremic rats. STS also has antioxidant activity, which can alleviate oxidative stress injury and improve the function of vascular endothelium. This is probably one of the mechanisms that can prevent calcification.

关 键 词：尿毒症大鼠 血管钙化 硫代硫酸钠 胎球蛋白A 

分 类 号：R972[医药卫生—药品]