PTEN、BMI-1、CIP2A、ALOX-5、TWIST-1及PML基因在慢性粒细胞白血病各分期表达差异  

Expressions of PTEN,BMI-1,CIP2A,ALOX-5,TWIST-1 and PML genes in chronic myelogenous leukemia

作　　者：钱伟[1] 杨明珍[1] 夏雷鸣[1] 郭婷婷[1] 赵雨辰[1] 谢研研 

机构地区：[1]安徽医科大学第一附属医院血液内科,合肥230022

出　　处：《安徽医科大学学报》2014年第11期1641-1646,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技厅自然科学基金(编号:11010402168)

摘　　要：目的探讨PTEN、BMI-1、CIP2A、ALOX-5、TWIST-1及PML基因在慢性粒细胞白血病(CML)患者疾病进展过程中的临床意义,同时初步评价Sokal积分在分子生物学水平预后效力。方法运用实时定量PCR法分别测定107例骨髓标本[CML慢性期(CP)患者64例,进展期患者23例,对照组20例]GAPDH、PTEN、BMI-1、CIP2A、ALOX-5、TWIST-1及PML基因相对表达量。分析CP、进展期包括加速期(AP)及急变期(BP)患者骨髓中上述基因表达水平差异。结果 BMI-1、CIP2A及TWIST-1基因在AP/BP组表达明显增高,与CP组相比差异有统计学意义(t=-4.576、-5.096、-2.714,P<0.05)。按Sokal积分进行危险度分层后,上述基因在各组间差异无统计学意义。结论 BMI-1、CIP2A及TWIST-1基因在CML进展过程中明显增高,提示与疾病进展有关。Sokal预后系统结合相关分子生物学指标评价预后或许是今后发展的方向。Objective To detect clinical significance of PTEN, BMI-1, CIP2A, ALOX-5, TWIST-1 and PML genes, in different phases of chronic myelogenous leukemia（ CML）, and evaluate the effeetion of prognosis of Sokal preliminarily. Methods The expression levels of GAPDH, PTEN, BMI-1, CIP2A, ALOX-5, TWIST-1 and PML were detected in 107 bone marrow samples （64 chronic phase patients,23 accelerated and blastic phase patients, 20 normals） by RT-PCR. The differences of expressions of these genes in different phases of CML were analyzed. Results Expressions of BMI-1 ,CIP2A and TWIST-1 in AP/BP were significantly higher than those in CP（t = - 4. 576, - 5. 096, - 2. 714, P 〈 0. 05 ）. These patients were analyzed by Sokal, the statistical differences between these genes and groups were not significant. Conclusion The increased expressions of BMI-1, CIP2A and TWIST- 1 maybe account partly for blast crisis of CML. The sokal contains molecular biological index may find direction in the future.

关 键 词：慢性粒细胞性白血病 Sokal积分 预后基因 荧光 定量PCR 

分 类 号：R557.1[医药卫生—血液循环系统疾病]