雌激素β受体在人椎间盘髓核细胞上的表达  

Expression and localization of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc

作　　者：陈鸿亮[1] 柯秀荣 楼超[1] 李游宇[2] 胡春飞[2] 郭彩云[2] 陈思思[2] 徐华梓[1] 

机构地区：[1]温州医科大学附属第二医院骨科,浙江温州325027 [2]温州医科大学第二临床医学院,浙江温州325035

出　　处：《温州医学院学报》2014年第9期646-648,共3页Journal of Wenzhou Medical College

基　　金：温州市科技局科研基金资助项目(Y20090272)

摘　　要：目的:研究雌激素β受体(ERβ)在人椎间盘髓核细胞上的表达。方法:7例椎间盘突出症患者,手术切除之髓核组织经体外消化、细胞培养,提取总RNA,RT-PCR检测ERβ基因的表达;另外通过免疫组织化学方法检测ERβ在人椎间盘髓核细胞中的分布。结果:7例样本RT-PCR扩增产物经琼脂糖凝胶电泳,在323 bp处有单一高亮条带;免疫组织化学方法亦证实细胞质中有ERβ的分布。结论:人椎间盘髓核细胞中存在ERβ的表达。Objective：To investigate the expression of estrogen receptor-βin nucleus pulposus cells of the human intervertebral disc. MethodSeven nucleus pulposus specimens were obtained from 7 patients during surgery. Cultured disc cells were used for immunocytochemical localization of ERβ. mRNA was isolated from cultured human disc cells, and RT-PCR ampliifcation of ERβwas employed to document molecular expression of this receptor. ResultExpression and localization of estrogen receptor-βwere detected in all 7 specimens. A goldview-stained agarose gel electrophoresis showing gene expression of ERβas a single band at the predicted 323 bp size. Localization was seen in the cytoplasm in all cultured disc cells with the immunocytochemistry method. Conclusion：This study demonstrates the expression of estrogen receptor-βin nucleus pulposus cells of the human intervertebral disc.

关 键 词：椎间盘 髓核 雌激素受体Β 

分 类 号：R681.5[医药卫生—骨科学]