温郁金乙酸乙酯提取物的抗炎镇痛作用  

Anti-flammatory and analgesic effects of ethyl acetate extract from Curcuma wenyujin

作　　者：裘关关[1] 蔡渊[1] 方亮莲[1] 林潍潍 董建勇[1] 

机构地区：[1]温州医科大学药学院,浙江温州325035

出　　处：《温州医学院学报》2014年第9期660-663,共4页Journal of Wenzhou Medical College

基　　金：浙江省大学生新苗人才计划项目(2013R413054)

摘　　要：目的:观察温郁金乙酸乙酯提取物的抗炎、镇痛作用。方法:采用小鼠热板法、二甲苯致耳肿胀法和冰醋酸致小鼠扭体实验等分析低剂量(100 mg·kg-1)、中剂量(200 mg·kg-1)、高剂量(400 mg·kg-1)温郁金乙酸乙酯提取物的抗炎、镇痛作用;采用ELISA法测定致炎耳组织中肿瘤坏死因子α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)的含量。结果:温郁金乙酸乙酯提取物对物理、化学因素引发的疼痛和致炎剂诱导的耳肿胀及毛细血管通透性改变等急性炎症具有明显的抑制作用,并随着剂量的增加作用增强,高剂量能明显降低耳组织中TNF-α的含量。结论:温郁金乙酸乙酯提取物具有显著的抗炎、镇痛作用,这一作用可能主要通过抑制TNF-α而实现。Objective：To investigate the analgesic and anti-lfammatory effects of the ethyl acetate extract of Curcuma wenyujin and explore the anti-lfammatory mechanism of this effective fraction. MethodBy using hot plate test, xylene-induced mouse ear edema test and chemical irritation-induced writhing test to study the effect of analgesic and anti-lfammatory. The levels of TNF-α, IL-1β, IL-6 in ear homogenate were measured by ELISA. ResultThe results showed that the ethyl acetate extract of Curcuma wenyujin had the significant analgesic effects by physical and chemical stimulated. It had obviously inhibited inlfammatory edema and celiac capillary permeability, the analgesic effects and anti-lfammatory was improved with increased dosages. It could also reduce the level of TNF-α. Conclusion：The ethyl acetate extract of Curcuma wenyujin have the signiifcant anti-lfamma-tory and analgesic effects, the mechanisms of anti-lfammatory is concerned with inhibiting the level of TNF-α.

关 键 词：温郁金 乙酸乙酯提取物 抗炎 镇痛 

分 类 号：R965.1[医药卫生—药理学]