1株产细菌纤维素芽胞杆菌的分离及鉴定  

Isolation and identification of bacterial cellulose producing strain

作　　者：朱宏阳[1] 姚俊[2] 冯珊[1] 李泳宁[1] 林伟铃[1] 

机构地区：[1]福建卫生职业技术学院药学系,福州350101 [2]南京医科大学基础医学院,南京210029

出　　处：《生物加工过程》2014年第5期80-84,共5页Chinese Journal of Bioprocess Engineering

基　　金：福建省自然科学青年基金(2012J05015);福建省教育厅A类科技项目(JA12430)

摘　　要：从残次水果中分离出1株菌株ZF-7,其产物经纤维素特异性染色反应、红外光谱分析及纤维素酶水解实验后,被确定为细菌纤维素。在对ZF-7菌株常规形态学及生理生化特性鉴定的基础上,对部分长度的16S rDNA同源性进行了分析,发现ZF-7菌株与解淀粉芽胞杆菌相似度可达99.5%,现命名为Bacillus amyloliquefaciens ZF-7。对ZF-7菌株在振荡培养和静置培养条件下的发酵性能进行了初步考察,得到细菌纤维素产率分别为6.6和6.2 g/L。Strain ZF-7 was isolated from bad fruits. Its product was determined as bacterial cellulose（BC） in view of the results of cellulose special reaction,FT-IR spectroscopy analysis and cellulose hydrolysis experiments. Based on morphology,physiological and biochemical characteristics,ZF-7 was identified by 16 S rDNA sequences and systematic analysis. The results indicated that 16 S rDNA sequences of ZF-7 had 99. 5% similarity to the partial sequence of Bacillus amyloliquefaciens,suggesting that ZF-7 was categorized as a subspecies of B. amyloliquefaciens, and denominated as B. amyloliquefaciens ZF-7. The fermentation performance of ZF-7 under shaking and static flasks was inspected preliminarily,and the BC production was 6. 6 and 6. 2 g /L,respectively.

关 键 词：细菌纤维素 解淀粉芽胞杆菌 筛选 鉴定 

分 类 号：Q93-331[生物学—微生物学]