罂粟科植物防御素的预测及生物信息学分析  

Prediction and Bioinformatic Analysis of Plant Defensins in Papaveraceae

作　　者：李骞[1] 许琪瑶 宋南[1] 龙可人 张学文[1] 陈金军[1] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128

出　　处：《作物研究》2014年第6期653-660,共8页Crop Research

基　　金：国家自然科学基金项目(30900242;31071943;31272339);湖南省自然科学基金项目(14JJ3093);湖南省教育厅项目(10B049);湖南省科技厅项目(2012NK4048;2013NK4003;2014NK3048);长沙市科技计划项目(K1307106-31)

摘　　要：在对博落回防御素研究的基础上,于NCBI数据库中系统搜索罂粟科所有已登录的EST序列文库,推测出31条新颖的罂粟科植物防御素。分析了这些防御素的相对分子质量、p I值、净电荷、疏水性平均值、蛋白结合能力、信号肽、亚细胞定位及其三级结构。Many researches focus on the medicinal value of alkaloids of Papaveraceae plants.However,the host non -spe-cific defense system,defensin,which contributes the broad -spectrum antimicrobial activity to the plants,are the same pre-cious for study.Based on the analysis of the cDNA clones of defensins we identified from Macleaya cordata,a species of Papaveraceae,31 novel defensins in Papaveraceae are identified by searching on the all registered Expressed Sequence Tags （ESTs）from the whole family Papaveraceae in the NCBI database.The molecular weight,pI value,net charge,the av-erage hydrophobicity,protein -binding Potential （Boman index）,signal peptide,subcellular localization and tertiary struc-ture of the novel identified defensins are predicted and analyzed.The properties of the defensins from Papaveraceae are summarized,and the genetic evolution are analyzed.This study provides a new perspective to study the antibacterial sub-stances in Papaveraceae.

关 键 词：罂粟科 防御素 生物信息学分析 

分 类 号：Q811.4[生物学—生物工程]