细菌全局性调控因子CsrA功能及其作用机制研究进展  

Research Progress on the Function and Regulatory Mechanism of CsrA,a Global Regulator in Bacteria

作　　者：尚立国[1] 战嵛华[1] 弓湃 燕永亮[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2014年第4期79-86,共8页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(31170081;31230004和31070084);中央级公益科研院所基本业务费专项;广东省引进创新创业团队计划项目(2013S033)资助

摘　　要：CsrA最初在研究碳储存调节系统(carbon storage regulation system)时被发现并被命名,是一个在肠杆菌、假单胞菌及芽孢杆菌等微生物中广泛存在的转录后调节因子,它可通过与靶标基因mRNA结合进而影响其稳定性或者抑制mRNA的翻译最终影响靶基因的表达。研究表明,CsrA属全局调控因子,不仅参与细菌的中心碳代谢、运动、生物膜形成、群体感应、致病性等多种生命活动,而且也参与了细菌多种次级代谢物的合成,具有重要的生物学意义。最近的研究表明,CsrA功能的实现与两个非编码调控RNA具有重要关联,它们组成了复杂而精细的调控单元。综述了不同微生物中CsrA功能的多样性,并阐述了CsrA参与分子调控的机制,对于深入认识碳储存系统的调控机制以及针对性的改造和利用该系统具有重要的理论意义。CsrA was initially found and named during research on the carbon storage regulatory system. CsrA affects stability and translation of target genes by binding mRNA as a widespread post-transcriptional regulator in Enterobacterium,Pseudomonas,Bacillus and other microorganisms. Studies indicated that CsrA not only regulated a number of physiological processes such as central carbon metabolism,motility,biofilm formation,quorum sensing,and pathogenicity,but also influenced the synthesis of many secondary metabolites. Recent research showed that the functions of CsrA were strictly related to two non-coding RNAs; these three genes coordinated to form a complex system. This review introduced the various functions of CsrA in different bacteria and its regulatory mechanism,information that will be useful for learning more about the regulatory mechanism of the carbon storage system and alter it for future studies.

关 键 词：碳储存调控系统 csrA基因 非编码RNA 群体感应 生物膜 

分 类 号：Q78[生物学—分子生物学]