透明质酸对环磷酰胺诱导膀胱炎大鼠前列腺素E2的影响  

Effect of hyaluronic acid on prostaglandin E2 in rats with cyclophosphamide-induced cystitis

作　　者：毛厚平[1] 吕夷松[1] 周辉良[1] 高锐[1] 江涛[1] 陈沁[1] 唐松喜 林曦[1] 曹林升[1] 

机构地区：[1]福建医科大学附属第一医院泌尿外科,福州350005

出　　处：《中华泌尿外科杂志》2014年第10期782-785,共4页Chinese Journal of Urology

基　　金：福建省教育厅青年基金（JA14148）

摘　　要：目的 探讨透明质酸膀胱灌注对环磷酰胺诱导膀胱炎中前列腺素E2（PGE2）的影响及意义. 方法 2012年6-12月SD大鼠18只,应用随机数字表随机分为对照组（生理盐水）、模型组（腹腔注射环磷酰胺）和干预组（透明质酸0.8 g/L膀胱灌注）,每组6只.酶联免疫吸附法检测尿液PGE2含量,vonFrey刷计算疼痛敏感性,代谢笼评价排尿习惯,HE染色观察炎症程度,甲苯胺蓝染色计数肥大细胞,免疫组化染色法检测膜结合型前列腺素E2合酶1（mPGES1）.比较各组指标差异,P＜0.05为差异有统计学意义. 结果 与对照组相比,模型组各项指标均明显升高（P＜0.05）,每视野炎症细胞及肥大细胞数目分别为39.50±8.31、21.42±3.94,24 h排尿次数32.55±5.74,PGE2含量（10.53±1.94） pg/ml,mPGES1吸光度达26.12±3.51.经透明质酸治疗,干预组各项指标均有明显改善（P＜0.05）,每视野炎症细胞及肥大细胞数目分别为17.20±5.13、10.11±1.85,24 h排尿次数21.32±3.59,PGE2含量（7.53±1.25） pg/ml,mPGES1吸光度为10.59±1.92. 结论 抑制mPGES1/PGE2可能是透明质酸治疗环磷酰胺诱导膀胱炎的途径之一.Objective To investigate the effect of hyaluronic acid on prostaglandin E2 （PGE2） in rats with cyclophosphamide-incuced cystitis.Methods From June 2012 to December 2012,18 SpragueDawley rats were randomized into control group （normal saline）,model group （intraperitoneal injection cyclophosphamide） and intervention group （intravesical 0.8 g/L hyaluronic acid） with 6 rats in each group.Urine PGE2 levels were evaluated by enzyme-linked immunosorbent assay,histological changes were evaluated by hematoxylin and eosin,toluidine blue and immunohistochemistry,and functional changes were evaluated by micturition habits and nociceptive behaviors.Results Compared with the control group,each items in the model group increased significantly （P〈0.05）.The counts of inflammatory cells and mast cells were 39.50±8.31 and 21.42±3.94 respectively and the number of 24 hours micturition was 32.55±5.74.The levels of PGE2 were （10.53 ± 1.94） pg/ml,and the A of mierosomal prostaglandin E2 synthase-1 （mPGES1） was 26.12±3.51.After treatment of hyaluronic acid,all items decreased significantly （P〈 0.05）.The counts of inflammatory cells and mast cells were 17.20± 5.13 and 10.11 ± 1.85 respectively.The number of 24 hours micturition was 21.32±3.59.The level of PGE2 was （7.53±1.25） pg/ml,and the A of mPGES1 was 10.59±1.92.Conclusion The inhibition of mPGES1/PGE2 might be one of the ways of hyaluronic acid in treatment of cyclophosphamide-induced cystitis.

关 键 词：膀胱炎 间质性 环磷酰胺 透明质酸 前列腺素E2 大鼠 

分 类 号：R694.3[医药卫生—泌尿科学]