TP-ELISA法检测梅毒抗体假阳性结果的影响因素  

Uncertain factors of detecting Treponema pallidum antibody by enzyme linked immunosorbent assay

作　　者：赵花[1] 李军民[1] 张红[1] 

机构地区：[1]武警陕西总队医院检验科,西安710054

出　　处：《武警医学》2014年第10期1003-1004,1007,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的：探讨TP-ELISA法检测梅毒特异性抗体假阳性结果的影响因素。方法采用TP-ELISA法对2619例标本进行检测，初检阳性者再行TRUST试验和TPPA确认试验，结果判定以TPPA为准，比较TP-ELISA法与TPPA法的符合率。结果231例TP-ELISA法检测阳性标本中S/CO值＞1．0者201例，经TRUST复检后阳性者169例，经TPPA确认阳性者196例，TP-ELISA法和TPPA的符合率为97．5％。在S/CO为0．70～1．0的30例标本中，经TRUST复检后阳性者12例，经TPPA确证为阳性者19例，TP-ELISA法和TPPA的符合率为63．3％。结论 TP-ELISA法检测阳性标本中S/CO值为0．70～1．0者应采用TRUST和 TPPA法联合检验，以减少误诊及漏检。Objective To study the uncertain factors of the false positive results of Treponema pallidum specific antibody（ anti-TP） detected by enzyme linked immunosorbent assay（ELISA）.Methods Anti-TP was detected in 2619 samples by ELISA.The pos-itive samples were examined again by tolulized red unheated serum test （ TRUST） and T.pallidum particle assay （ TPPA） .Results The S/CO〉1.0 in 231 positive samples detected by ELISA were 201, The 201 positive samples were examined again by TRUST and confirmed positive by TPPA.The positive samples by TRUST were 169, and by TPPA were 196.The coincidence rate of ELISA and TPPA was 97.5%.In 30 samples’ S/CO between 0.7 and 1.0, the positive samples examined again by TRUST were 12, and con-firmed positive by TPPA were 19.The coincidence rate of ELISA and TPPA was 63.3%.Conclusions The method of ELISA is af-fected by the uncertain factors, So false positive results exist.The sample’ s S/CO between 0.7 and 1.0 should be examined by TRUST and TPPA jointly.

关 键 词：梅毒螺旋体 特异性抗体检测 非特异性抗体检测 确认试验 

分 类 号：R446.111[医药卫生—诊断学] R759.1[医药卫生—临床医学]