下瘀血汤中大黄生熟互换对热结血瘀模型大鼠血管内皮功能及微循环的影响  

Effect of raw/stewed Rhubarb in Xiayuxue Decoction on vascular endothelial function and microcirculation of rats with heat accumulation of blood stasis

作　　者：赵玲[1] 胡昌江[1] 潘新[1] 耿媛媛[1] 吴晓青[1] 吴文辉[1] 张乐[1] 

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《药物评价研究》2014年第5期413-416,共4页Drug Evaluation Research

基　　金：中医药行业科研专项(20110700710)

摘　　要：目的研究下瘀血汤中大黄生熟互换对热结血瘀模型大鼠血管内皮功能及微循环的影响。方法采用ig热性中药结合sc盐酸肾上腺素的方法,复制热结血瘀大鼠模型。大鼠ig热性中药28 d,第22天起sc盐酸肾上腺素,并ig相应的药物。第29天,眼眶取血检测检测血管内皮素(ET)、一氧化氮(NO)、前列环素(PGI2)、血管性血友病因子(vWF);股动脉取血,检测血液流变学各项指标。结果含生大黄(复方A)和熟大黄(复方B)下瘀血汤均具有改善热结血瘀模型大鼠血液流变学、血管内皮细胞损伤和微循环的作用;与复方A相比,复方B各剂量组对血液流变学各指标、ET、NO、PGI2、vWF因子水平具有显著的改善作用(P<0.05、0.01)。结论含熟大黄下瘀血汤的活血化瘀作用更强,可能是通过调节ET、NO、PGI2、vWF因子水平而实现的。Objective To study the effect of raw/stewed Rhubarb in Xiayuxue Decoction on vascular endothelial function and microcirculation of rats with heat accumulation of blood stasis Methods Seventy SD rats were divided into seven groups. The rats in experimental groups were given heat drugs of traditional Chinese medicine for 28 d, then injected adrenaline hydrochloride for later 7 d, twice a day. At the same time, those experimental rats were given different doses of Xiayuxue Decoction （9 and 4.5 g ＂kg-1） orally for 7 d. Then blood rheologic parameters such as ET, NO, PGI2, and VWF in rats were detected. Results Xiayuxue Decoction A/B have improved vascular endothelial cell injury and the microcirculation of the rats with heat accumulation of blood stasis. Compared with Xiayuxue Decoction A, each dose of Xiayuxue Decoction B has improved the levels of ET, NO, PGI2, and vWF factor significantly （P 〈 0.05, 0.01）. The efficiency on promoting blood circulation to remove blood stasis of Xiayuxue Decoction B was definitely better than Xiayuxue Decoction A. Conclusion The mechanism of promoting blood circulation to remove blood stasis may be through regulating the levels of ET, NO, PGI2, and VWF factor.

关 键 词：下瘀血汤 生/熟大黄 热结血瘀模型 ET NO PGI2 VWF因子 

分 类 号：R285[医药卫生—中药学]